Worldwide genotyping of castor bean germplasm (Ricinus communis L.) using AFLPs and SSRs

Gerard J Allan, Amber Williams, Pablo D. Rabinowicz, Agnes P. Chan, Jacques Ravel, Paul S Keim

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Worldwide genetic diversity in 200 individuals comprising 41 castor bean accessions was assessed using amplified fragment polymorphisms (AFLPs) and simple sequence repeats (SSRs). We found that, despite surveying five continents and 35 countries, genetic diversity in castor bean germplasm is relatively low (overall H e = 0.126 for AFLPs and 0.188 for SSRs) compared to estimates of genetic diversity in other plant species. Our data also show no geographic structuring of genotypes across continents or countries within continents. An assessment of the congruence between AFLP and SSRs indicates a low correlation (R 2 = 0.19) between the two data sets, but each marker class nonetheless shows similar patterns of low-genetic diversity and a lack of geographic structure. Our data do suggest that SSRs yield a higher percentage of polymorphic loci, higher heterozyosity and a greater range of genetic distances, and are therefore more informative than are AFLPs on a locus-by-locus basis. Based on comparisons with numerous other plant species, we suggest that the lower genetic variation in this worldwide collection may be due to one or more factors including: sampling strategies that have not captured the full extent of genetic variation in the species; artifactual variation due to long-term germplasm storage and seed regeneration; or intense selection followed by domestic cultivation of a limited number of castor bean genotypes, which are widely propagated for their horticultural and agro-economic value.

Original languageEnglish (US)
Pages (from-to)365-378
Number of pages14
JournalGenetic Resources and Crop Evolution
Volume55
Issue number3
DOIs
StatePublished - May 2008

Fingerprint

Castor Bean
Ricinus
castor beans
Ricinus communis
germplasm
Microsatellite Repeats
genotyping
polymorphism
genetic polymorphism
microsatellite repeats
genetic variation
genotype
loci
Genotype
surveying
regeneration
seed
economic valuation
genetic distance
genetic diversity

Keywords

  • Amplified fragment length polymorphisms
  • Ricinus communis
  • Simple sequence repeats

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Worldwide genotyping of castor bean germplasm (Ricinus communis L.) using AFLPs and SSRs. / Allan, Gerard J; Williams, Amber; Rabinowicz, Pablo D.; Chan, Agnes P.; Ravel, Jacques; Keim, Paul S.

In: Genetic Resources and Crop Evolution, Vol. 55, No. 3, 05.2008, p. 365-378.

Research output: Contribution to journalArticle

Allan, Gerard J ; Williams, Amber ; Rabinowicz, Pablo D. ; Chan, Agnes P. ; Ravel, Jacques ; Keim, Paul S. / Worldwide genotyping of castor bean germplasm (Ricinus communis L.) using AFLPs and SSRs. In: Genetic Resources and Crop Evolution. 2008 ; Vol. 55, No. 3. pp. 365-378.
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