Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis

Erin P. Price, Heidie M. Hornstra, Direk Limmathurotsakul, Tamara L. Max, Derek S. Sarovich, Amy J. Vogler, Julia L. Dale, Jennifer L. Ginther, Benjamin Leadem, Rebecca E. Colman, Jeffrey T Foster, Apichai Tuanyok, David M Wagner, Sharon J. Peacock, Talima R Pearson, Paul S Keim

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Little is currently known about bacterial pathogen evolution and adaptation within the host during acute infection. Previous studies of Burkholderia pseudomallei, the etiologic agent of melioidosis, have shown that this opportunistic pathogen mutates rapidly both in vitro and in vivo at tandemly repeated loci, making this organism a relevant model for studying short-term evolution. In the current study, B. pseudomallei isolates cultured from multiple body sites from four Thai patients with disseminated melioidosis were subjected to fine-scale genotyping using multilocus variable-number tandem repeat analysis (MLVA). In order to understand and model the in vivo variable-number tandem repeat (VNTR) mutational process, we characterized the patterns and rates of mutations in vitro through parallel serial passage experiments of B. pseudomallei. Despite the short period of infection, substantial divergence from the putative founder genotype was observed in all four melioidosis cases. This study presents a paradigm for examining bacterial evolution over the short timescale of an acute infection. Further studies are required to determine whether the mutational process leads to phenotypic alterations that impact upon bacterial fitness in vivo. Our findings have important implications for future sampling strategies, since colonies in a single clinical sample may be genetically heterogeneous, and organisms in a culture taken late in the infective process may have undergone considerable genetic change compared with the founder inoculum.

Original languageEnglish (US)
Article numbere1000725
JournalPLoS Pathogens
Volume6
Issue number1
DOIs
StatePublished - Jan 2010

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Melioidosis
Burkholderia pseudomallei
Minisatellite Repeats
Infection
Serial Passage
Mutation Rate
Genotype
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Price, E. P., Hornstra, H. M., Limmathurotsakul, D., Max, T. L., Sarovich, D. S., Vogler, A. J., ... Keim, P. S. (2010). Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis. PLoS Pathogens, 6(1), [e1000725]. https://doi.org/10.1371/journal.ppat.1000725

Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis. / Price, Erin P.; Hornstra, Heidie M.; Limmathurotsakul, Direk; Max, Tamara L.; Sarovich, Derek S.; Vogler, Amy J.; Dale, Julia L.; Ginther, Jennifer L.; Leadem, Benjamin; Colman, Rebecca E.; Foster, Jeffrey T; Tuanyok, Apichai; Wagner, David M; Peacock, Sharon J.; Pearson, Talima R; Keim, Paul S.

In: PLoS Pathogens, Vol. 6, No. 1, e1000725, 01.2010.

Research output: Contribution to journalArticle

Price, EP, Hornstra, HM, Limmathurotsakul, D, Max, TL, Sarovich, DS, Vogler, AJ, Dale, JL, Ginther, JL, Leadem, B, Colman, RE, Foster, JT, Tuanyok, A, Wagner, DM, Peacock, SJ, Pearson, TR & Keim, PS 2010, 'Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis', PLoS Pathogens, vol. 6, no. 1, e1000725. https://doi.org/10.1371/journal.ppat.1000725
Price EP, Hornstra HM, Limmathurotsakul D, Max TL, Sarovich DS, Vogler AJ et al. Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis. PLoS Pathogens. 2010 Jan;6(1). e1000725. https://doi.org/10.1371/journal.ppat.1000725
Price, Erin P. ; Hornstra, Heidie M. ; Limmathurotsakul, Direk ; Max, Tamara L. ; Sarovich, Derek S. ; Vogler, Amy J. ; Dale, Julia L. ; Ginther, Jennifer L. ; Leadem, Benjamin ; Colman, Rebecca E. ; Foster, Jeffrey T ; Tuanyok, Apichai ; Wagner, David M ; Peacock, Sharon J. ; Pearson, Talima R ; Keim, Paul S. / Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis. In: PLoS Pathogens. 2010 ; Vol. 6, No. 1.
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