Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011

Kizee A. Etienne, John Gillece, Remy Hilsabeck, Jim M. Schupp, Rebecca Colman, Shawn R. Lockhart, Lalitha Gade, Elizabeth H. Thompson, Deanna A. Sutton, Robyn Neblett-Fanfair, Benjamin J. Park, George Turabelidze, Paul S Keim, Mary E. Brandt, Eszter Deak, David M. Engelthaler

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces.

Original languageEnglish (US)
Article numbere49989
JournalPLoS One
Volume7
Issue number11
DOIs
StatePublished - Nov 27 2012
Externally publishedYes

Fingerprint

Tornadoes
tornadoes
Disease Outbreaks
Genes
Genome
genome
Single Nucleotide Polymorphism
phylogeny
Genotype
lacerations
Lacerations
genotype
Spores
Debris
polluted soils
linkage (genetics)
Sequence Analysis
population structure
Soil
spores

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Etienne, K. A., Gillece, J., Hilsabeck, R., Schupp, J. M., Colman, R., Lockhart, S. R., ... Engelthaler, D. M. (2012). Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011. PLoS One, 7(11), [e49989]. https://doi.org/10.1371/journal.pone.0049989

Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011. / Etienne, Kizee A.; Gillece, John; Hilsabeck, Remy; Schupp, Jim M.; Colman, Rebecca; Lockhart, Shawn R.; Gade, Lalitha; Thompson, Elizabeth H.; Sutton, Deanna A.; Neblett-Fanfair, Robyn; Park, Benjamin J.; Turabelidze, George; Keim, Paul S; Brandt, Mary E.; Deak, Eszter; Engelthaler, David M.

In: PLoS One, Vol. 7, No. 11, e49989, 27.11.2012.

Research output: Contribution to journalArticle

Etienne, KA, Gillece, J, Hilsabeck, R, Schupp, JM, Colman, R, Lockhart, SR, Gade, L, Thompson, EH, Sutton, DA, Neblett-Fanfair, R, Park, BJ, Turabelidze, G, Keim, PS, Brandt, ME, Deak, E & Engelthaler, DM 2012, 'Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011', PLoS One, vol. 7, no. 11, e49989. https://doi.org/10.1371/journal.pone.0049989
Etienne, Kizee A. ; Gillece, John ; Hilsabeck, Remy ; Schupp, Jim M. ; Colman, Rebecca ; Lockhart, Shawn R. ; Gade, Lalitha ; Thompson, Elizabeth H. ; Sutton, Deanna A. ; Neblett-Fanfair, Robyn ; Park, Benjamin J. ; Turabelidze, George ; Keim, Paul S ; Brandt, Mary E. ; Deak, Eszter ; Engelthaler, David M. / Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011. In: PLoS One. 2012 ; Vol. 7, No. 11.
@article{da89e40c319046ac8f64d5ab77aad038,
title = "Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011",
abstract = "Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces.",
author = "Etienne, {Kizee A.} and John Gillece and Remy Hilsabeck and Schupp, {Jim M.} and Rebecca Colman and Lockhart, {Shawn R.} and Lalitha Gade and Thompson, {Elizabeth H.} and Sutton, {Deanna A.} and Robyn Neblett-Fanfair and Park, {Benjamin J.} and George Turabelidze and Keim, {Paul S} and Brandt, {Mary E.} and Eszter Deak and Engelthaler, {David M.}",
year = "2012",
month = "11",
day = "27",
doi = "10.1371/journal.pone.0049989",
language = "English (US)",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011

AU - Etienne, Kizee A.

AU - Gillece, John

AU - Hilsabeck, Remy

AU - Schupp, Jim M.

AU - Colman, Rebecca

AU - Lockhart, Shawn R.

AU - Gade, Lalitha

AU - Thompson, Elizabeth H.

AU - Sutton, Deanna A.

AU - Neblett-Fanfair, Robyn

AU - Park, Benjamin J.

AU - Turabelidze, George

AU - Keim, Paul S

AU - Brandt, Mary E.

AU - Deak, Eszter

AU - Engelthaler, David M.

PY - 2012/11/27

Y1 - 2012/11/27

N2 - Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces.

AB - Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces.

UR - http://www.scopus.com/inward/record.url?scp=84870276865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870276865&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0049989

DO - 10.1371/journal.pone.0049989

M3 - Article

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e49989

ER -