White-Nose Syndrome Disease Severity and a Comparison of Diagnostic Methods

Liam P. McGuire, James M. Turner, Lisa Warnecke, Glenna McGregor, Trent K. Bollinger, Vikram Misra, Jeffrey T Foster, Winifred F. Frick, A. Marm Kilpatrick, Craig K R Willis

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

White-nose syndrome is caused by the fungus Pseudogymnoascus destructans and has killed millions of hibernating bats in North America but the pathophysiology of the disease remains poorly understood. Our objectives were to (1) assess non-destructive diagnostic methods for P. destructans infection compared to histopathology, the current gold-standard, and (2) to evaluate potential metrics of disease severity. We used data from three captive inoculation experiments involving 181 little brown bats (Myotis lucifugus) to compare histopathology, quantitative PCR (qPCR), and ultraviolet fluorescence as diagnostic methods of P. destructans infection. To assess disease severity, we considered two histology metrics (wing area with fungal hyphae, area of dermal necrosis), P. destructans fungal load (qPCR), ultraviolet fluorescence, and blood chemistry (hematocrit, sodium, glucose, pCO2, and bicarbonate). Quantitative PCR was most effective for early detection of P. destructans, while all three methods were comparable in severe infections. Correlations among hyphae and necrosis scores, qPCR, ultraviolet fluorescence, blood chemistry, and hibernation duration indicate a multi-stage pattern of disease. Disruptions of homeostasis occurred rapidly in late hibernation. Our results provide valuable information about the use of non-destructive techniques for monitoring, and provide novel insight into the pathophysiology of white-nose syndrome, with implications for developing and implementing potential mitigation strategies.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalEcoHealth
DOIs
StateAccepted/In press - Mar 8 2016
Externally publishedYes

Fingerprint

Nose Diseases
disease severity
hibernation
fluorescence
histopathology
bat
Hibernation
Polymerase Chain Reaction
Hyphae
Fluorescence
blood
Nose
Necrosis
Infection
histology
homeostasis
bicarbonate
inoculation
glucose
mitigation

Keywords

  • blood chemistry
  • histopathology
  • Myotis lucifugus
  • non-destructive methods
  • PCR
  • Pseudogymnoascus destructans
  • ultraviolet fluorescence

ASJC Scopus subject areas

  • Ecology
  • Health, Toxicology and Mutagenesis

Cite this

McGuire, L. P., Turner, J. M., Warnecke, L., McGregor, G., Bollinger, T. K., Misra, V., ... Willis, C. K. R. (Accepted/In press). White-Nose Syndrome Disease Severity and a Comparison of Diagnostic Methods. EcoHealth, 1-12. https://doi.org/10.1007/s10393-016-1107-y

White-Nose Syndrome Disease Severity and a Comparison of Diagnostic Methods. / McGuire, Liam P.; Turner, James M.; Warnecke, Lisa; McGregor, Glenna; Bollinger, Trent K.; Misra, Vikram; Foster, Jeffrey T; Frick, Winifred F.; Kilpatrick, A. Marm; Willis, Craig K R.

In: EcoHealth, 08.03.2016, p. 1-12.

Research output: Contribution to journalArticle

McGuire, LP, Turner, JM, Warnecke, L, McGregor, G, Bollinger, TK, Misra, V, Foster, JT, Frick, WF, Kilpatrick, AM & Willis, CKR 2016, 'White-Nose Syndrome Disease Severity and a Comparison of Diagnostic Methods', EcoHealth, pp. 1-12. https://doi.org/10.1007/s10393-016-1107-y
McGuire LP, Turner JM, Warnecke L, McGregor G, Bollinger TK, Misra V et al. White-Nose Syndrome Disease Severity and a Comparison of Diagnostic Methods. EcoHealth. 2016 Mar 8;1-12. https://doi.org/10.1007/s10393-016-1107-y
McGuire, Liam P. ; Turner, James M. ; Warnecke, Lisa ; McGregor, Glenna ; Bollinger, Trent K. ; Misra, Vikram ; Foster, Jeffrey T ; Frick, Winifred F. ; Kilpatrick, A. Marm ; Willis, Craig K R. / White-Nose Syndrome Disease Severity and a Comparison of Diagnostic Methods. In: EcoHealth. 2016 ; pp. 1-12.
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