When outgroups fail; Phylogenomics of rooting the emerging pathogen, Coxiella burnetii

Talima R Pearson, Heidie M. Hornstra, Jason W. Sahl, Sarah Schaack, James M. Schupp, Stephen M Beckstrom-Sternberg, Matthew W. O'Neill, Rachael A. Priestley, Mia D. Champion, James S. Beckstrom-Sternberg, Gilbert J. Kersh, James E. Samuel, Robert F. Massung, Paul S Keim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Rooting phylogenies is critical for understanding evolution, yet the importance, intricacies and difficulties of rooting are often overlooked. For rooting, polymorphic characters among the group of interest (ingroup) must be compared to those of a relative (outgroup) that diverged before the last common ancestor (LCA) of the ingroup. Problems arise if an outgroup does not exist, is unknown, or is so distant that few characters are shared, in which case duplicated genes originating before the LCA can be used as proxy outgroups to root diverse phylogenies. Here, we describe a genome-wide expansion of this technique that can be used to solve problems at the other end of the evolutionary scale: where ingroup individuals are all very closely related to each other, but the next closest relative is very distant. We used shared orthologous single nucleotide polymorphisms (SNPs) from 10 whole genome sequences of Coxiella burnetii, the causative agent of Q fever in humans, to create a robust, but unrooted phylogeny. To maximize the number of characters informative about the rooting, we searched entire genomes for polymorphic duplicated regions where orthologs of each paralog could be identified so that the paralogs could be used to root the tree. Recent radiations, such as those of emerging pathogens, often pose rooting challenges due to a lack of ingroup variation and large genomic differences with known outgroups. Using a phylogenomic approach, we created a robust, rooted phylogeny for C. burnetii. [Coxiella burnetii; paralog SNPs; pathogen evolution; phylogeny; recent radiation; root; rooting using duplicated genes.]

Original languageEnglish (US)
Pages (from-to)752-762
Number of pages11
JournalSystematic Biology
Volume62
Issue number5
DOIs
StatePublished - Sep 2013

Fingerprint

Coxiella burnetii
Phylogeny
rooting
pathogen
phylogeny
pathogens
Genome
genome
Single Nucleotide Polymorphism
common ancestry
single nucleotide polymorphism
Radiation
ancestry
polymorphism
Q Fever
Public Opinion
Q fever
Proxy
gene
Genes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

When outgroups fail; Phylogenomics of rooting the emerging pathogen, Coxiella burnetii. / Pearson, Talima R; Hornstra, Heidie M.; Sahl, Jason W.; Schaack, Sarah; Schupp, James M.; Beckstrom-Sternberg, Stephen M; O'Neill, Matthew W.; Priestley, Rachael A.; Champion, Mia D.; Beckstrom-Sternberg, James S.; Kersh, Gilbert J.; Samuel, James E.; Massung, Robert F.; Keim, Paul S.

In: Systematic Biology, Vol. 62, No. 5, 09.2013, p. 752-762.

Research output: Contribution to journalArticle

Pearson, TR, Hornstra, HM, Sahl, JW, Schaack, S, Schupp, JM, Beckstrom-Sternberg, SM, O'Neill, MW, Priestley, RA, Champion, MD, Beckstrom-Sternberg, JS, Kersh, GJ, Samuel, JE, Massung, RF & Keim, PS 2013, 'When outgroups fail; Phylogenomics of rooting the emerging pathogen, Coxiella burnetii', Systematic Biology, vol. 62, no. 5, pp. 752-762. https://doi.org/10.1093/sysbio/syt038
Pearson, Talima R ; Hornstra, Heidie M. ; Sahl, Jason W. ; Schaack, Sarah ; Schupp, James M. ; Beckstrom-Sternberg, Stephen M ; O'Neill, Matthew W. ; Priestley, Rachael A. ; Champion, Mia D. ; Beckstrom-Sternberg, James S. ; Kersh, Gilbert J. ; Samuel, James E. ; Massung, Robert F. ; Keim, Paul S. / When outgroups fail; Phylogenomics of rooting the emerging pathogen, Coxiella burnetii. In: Systematic Biology. 2013 ; Vol. 62, No. 5. pp. 752-762.
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