Viability of Yersinia pestis subcultures in agar stabs

N. C. Leal, M. Sobreira, A. F Q Araújo, J. L O Magalhães, A. J. Vogler, M. C. Bollig, R. Nottingham, Paul S Keim, David M Wagner, A. M P Almeida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Since its identification as the causative agent of plague in 1894, thousands of Yersinia pestis strains have been isolated and stored. Here, we report the ability of Y. pestis to survive up to 47 years in agar stabs, in rubber-stoppered tubes, under refrigeration (+4 to +10°C), although overall subculture recovery rates were poor and inversely related to the length of time stored. Genetic characterization of virulence gene presence among these subcultures was suggestive of significant variation in the genomic stability of Y. pestis subcultures stored under these conditions. Specifically, we found variation in the presence of plasmid and chromosomal virulence markers (genes pla, lcrV, caf1 and irp2) among multiple subcultures of Y. pestis strains in the 'Collection of Yersinia pestis' (Fiocruz-CYP) maintained by the SRP of FIOCRUZ-PE in Brazil. This variation, together with all of the inherent temporal, geographic and other genetic variation represented by all of the recoverable strains in this historical collection was preserved in new frozen culture stocks stored at -70°C as a result of this study. These frozen culture stocks represent a valuable resource for future comparative studies of Y. pestis.

Original languageEnglish (US)
Pages (from-to)91-95
Number of pages5
JournalLetters in Applied Microbiology
Volume62
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Yersinia pestis
Agar
Virulence
Refrigeration
Plague
Genomic Instability
Rubber
Genes
Brazil
Plasmids

Keywords

  • Yersinia pestis
  • Collection
  • Genomic stability
  • Plague
  • Viability

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Leal, N. C., Sobreira, M., Araújo, A. F. Q., Magalhães, J. L. O., Vogler, A. J., Bollig, M. C., ... Almeida, A. M. P. (2016). Viability of Yersinia pestis subcultures in agar stabs. Letters in Applied Microbiology, 62(1), 91-95. https://doi.org/10.1111/lam.12519

Viability of Yersinia pestis subcultures in agar stabs. / Leal, N. C.; Sobreira, M.; Araújo, A. F Q; Magalhães, J. L O; Vogler, A. J.; Bollig, M. C.; Nottingham, R.; Keim, Paul S; Wagner, David M; Almeida, A. M P.

In: Letters in Applied Microbiology, Vol. 62, No. 1, 01.01.2016, p. 91-95.

Research output: Contribution to journalArticle

Leal, NC, Sobreira, M, Araújo, AFQ, Magalhães, JLO, Vogler, AJ, Bollig, MC, Nottingham, R, Keim, PS, Wagner, DM & Almeida, AMP 2016, 'Viability of Yersinia pestis subcultures in agar stabs', Letters in Applied Microbiology, vol. 62, no. 1, pp. 91-95. https://doi.org/10.1111/lam.12519
Leal NC, Sobreira M, Araújo AFQ, Magalhães JLO, Vogler AJ, Bollig MC et al. Viability of Yersinia pestis subcultures in agar stabs. Letters in Applied Microbiology. 2016 Jan 1;62(1):91-95. https://doi.org/10.1111/lam.12519
Leal, N. C. ; Sobreira, M. ; Araújo, A. F Q ; Magalhães, J. L O ; Vogler, A. J. ; Bollig, M. C. ; Nottingham, R. ; Keim, Paul S ; Wagner, David M ; Almeida, A. M P. / Viability of Yersinia pestis subcultures in agar stabs. In: Letters in Applied Microbiology. 2016 ; Vol. 62, No. 1. pp. 91-95.
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