Validating Whole Slide Digital Morphometric Analysis as a Microscopy Tool

Robert B. Diller, Robert S Kellar

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Whole slide imaging (WSI) can be used to quantify multiple responses within tissue sections during histological analysis. Feature Analysis on Consecutive Tissue Sections (FACTS®) allows the investigator to perform digital morphometric analysis (DMA) within specified regions of interest (ROI) across multiple serial sections at faster rates when compared with manual morphometry methods. Using FACTS® in conjunction with WSI is a powerful analysis tool, which allows DMA to target specific ROI across multiple tissue sections stained for different biomarkers. DMA may serve as an appropriate alternative to classic, manual, histologic morphometric measures, which have historically relied on the selection of high-powered fields of views and manual scoring (e.g., a gold standard). In the current study, existing preserved samples were used to determine if DMA would provide similar results to manual counting methods. Rodent hearts (n = 14, left ventricles) were stained with Masson's trichrome, and reacted for cluster of differentiation 68 (CD-68). This study found no statistical significant difference between a classic, manual method and the use of digital algorithms to perform the similar counts (p = 0.38). DMA offers researchers the ability to accurately evaluate morphological characteristics in a reproducible fashion without investigator bias and with higher throughput.

Original languageEnglish (US)
Pages (from-to)249-255
Number of pages7
JournalMicroscopy and Microanalysis
Volume21
Issue number1
DOIs
StatePublished - Jun 20 2014

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chutes
Microscopic examination
Tissue
microscopy
Imaging techniques
Biomarkers
rodents
scoring
biomarkers
Throughput
field of view
counting

Keywords

  • digital analysis
  • digital morphometric analysis
  • digital pathology
  • whole slide analysis
  • whole slide quantitative analysis

ASJC Scopus subject areas

  • Instrumentation

Cite this

Validating Whole Slide Digital Morphometric Analysis as a Microscopy Tool. / Diller, Robert B.; Kellar, Robert S.

In: Microscopy and Microanalysis, Vol. 21, No. 1, 20.06.2014, p. 249-255.

Research output: Contribution to journalArticle

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