Using QIIME to analyze 16s rRNA gene sequences from microbial communities

Justin Kuczynski; Jesse Stombaugh; William Anton Walters; Antonio González; James G Caporaso; Rob Knight

doi:10.1002/9780471729259.mc01e05s27

Using QIIME to analyze 16s rRNA gene sequences from microbial communities

Justin Kuczynski, Jesse Stombaugh, William Anton Walters, Antonio González, James G Caporaso, Rob Knight

Electrical Engineering and Computer Science

Research output: Contribution to journal › Article

237 Scopus citations

Abstract

QIIME (canonically pronounced "chime") is a software application that performs microbial community analysis. It is an acronym for Quantitative Insights Into Microbial Ecology, and has been used to analyze and interpret nucleic acid sequence data from fungal, viral, bacterial, and archaeal communities. The following protocols describe how to install QIIME on a single computer and use it to analyze microbial 16S sequence data from nine distinct microbial communities. Curr. Protoc. Microbiol.

Original language	English (US)
Article number	1E.5
Journal	Current Protocols in Microbiology
Issue number	SUPPL.27
DOIs	https://doi.org/10.1002/9780471729259.mc01e05s27
State	Published - 2012

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Keywords

16S
Bioinformatics
Microbial ecology
QIIME
Software
SSU

ASJC Scopus subject areas

Microbiology
Parasitology
Virology

Cite this

Kuczynski, J., Stombaugh, J., Walters, W. A., González, A., Caporaso, J. G., & Knight, R. (2012). Using QIIME to analyze 16s rRNA gene sequences from microbial communities. Current Protocols in Microbiology, (SUPPL.27), [1E.5]. https://doi.org/10.1002/9780471729259.mc01e05s27

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Access to Document

10.1002/9780471729259.mc01e05s27