Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

W. Ryan Easterday, Matthew N. Van Ert, Tatum S. Simonson, David M Wagner, Leo J. Kenefic, Christopher J. Allender, Paul S Keim

55 Scopus citations

Abstract

A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

Original languageEnglish (US)
Pages (from-to)1995-1997
Number of pages3
JournalJournal of Clinical Microbiology
Volume43
Issue number4
DOIs
StatePublished - Apr 2005

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