Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

W. Ryan Easterday, Matthew N. Van Ert, Tatum S. Simonson, David M. Wagner, Leo J. Kenefic, Christopher J. Allender, Paul Keim

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

Original languageEnglish (US)
Pages (from-to)1995-1997
Number of pages3
JournalJournal of Clinical Microbiology
Volume43
Issue number4
DOIs
StatePublished - Apr 1 2005

ASJC Scopus subject areas

  • Microbiology (medical)

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