Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis

W. Ryan Easterday, Matthew N. Van Ert, Tatum S. Simonson, David M Wagner, Leo J. Kenefic, Christopher J. Allender, Paul S Keim

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

Original languageEnglish (US)
Pages (from-to)1995-1997
Number of pages3
JournalJournal of Clinical Microbiology
Volume43
Issue number4
DOIs
StatePublished - Apr 2005

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Bacillus anthracis
Single Nucleotide Polymorphism
Nonsense Codon
Genes
Genotype

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis. / Easterday, W. Ryan; Van Ert, Matthew N.; Simonson, Tatum S.; Wagner, David M; Kenefic, Leo J.; Allender, Christopher J.; Keim, Paul S.

In: Journal of Clinical Microbiology, Vol. 43, No. 4, 04.2005, p. 1995-1997.

Research output: Contribution to journalArticle

Easterday, W. Ryan ; Van Ert, Matthew N. ; Simonson, Tatum S. ; Wagner, David M ; Kenefic, Leo J. ; Allender, Christopher J. ; Keim, Paul S. / Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis. In: Journal of Clinical Microbiology. 2005 ; Vol. 43, No. 4. pp. 1995-1997.
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