Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

Jana M. U'Ren, Matthew N. Van Ert, James M. Schupp, W. Ryan Easterday, Tatum S. Simonson, Richard T. Okinaka, Talima R Pearson, Paul S Keim

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

Original languageEnglish (US)
Pages (from-to)5771-5774
Number of pages4
JournalJournal of Clinical Microbiology
Volume43
Issue number11
DOIs
StatePublished - Nov 2005

Fingerprint

Burkholderia mallei
Burkholderia pseudomallei
Burkholderia
Burkholderia cepacia
Single Nucleotide Polymorphism
Real-Time Polymerase Chain Reaction
Genotype
Bacteria

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei. / U'Ren, Jana M.; Van Ert, Matthew N.; Schupp, James M.; Easterday, W. Ryan; Simonson, Tatum S.; Okinaka, Richard T.; Pearson, Talima R; Keim, Paul S.

In: Journal of Clinical Microbiology, Vol. 43, No. 11, 11.2005, p. 5771-5774.

Research output: Contribution to journalArticle

U'Ren, Jana M. ; Van Ert, Matthew N. ; Schupp, James M. ; Easterday, W. Ryan ; Simonson, Tatum S. ; Okinaka, Richard T. ; Pearson, Talima R ; Keim, Paul S. / Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei. In: Journal of Clinical Microbiology. 2005 ; Vol. 43, No. 11. pp. 5771-5774.
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