Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol

Mary E. Kauffman, William K. Keener, Scott R. Clingenpeel, Maribeth E Watwood, David W. Reed, Yoshiko Fujita, R. Michael Lehman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

3-Hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.

Original languageEnglish (US)
Pages (from-to)801-805
Number of pages5
JournalJournal of Microbiological Methods
Volume55
Issue number3
DOIs
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Toluene
Bacteria
Enzymes
3-methylcatechol

Keywords

  • 3-Hydroxyphenylacetylene
  • Activity
  • Chromogenic
  • Fluorogenic
  • Phenylacetylene
  • Toluene

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol. / Kauffman, Mary E.; Keener, William K.; Clingenpeel, Scott R.; Watwood, Maribeth E; Reed, David W.; Fujita, Yoshiko; Lehman, R. Michael.

In: Journal of Microbiological Methods, Vol. 55, No. 3, 12.2003, p. 801-805.

Research output: Contribution to journalArticle

Kauffman, Mary E. ; Keener, William K. ; Clingenpeel, Scott R. ; Watwood, Maribeth E ; Reed, David W. ; Fujita, Yoshiko ; Lehman, R. Michael. / Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol. In: Journal of Microbiological Methods. 2003 ; Vol. 55, No. 3. pp. 801-805.
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AU - Fujita, Yoshiko

AU - Lehman, R. Michael

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