Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol

Mary E. Kauffman, William K. Keener, Scott R. Clingenpeel, Mary E. Watwood, David W. Reed, Yoshiko Fujita, R. Michael Lehman

Research output: Contribution to journalArticle

11 Scopus citations


3-Hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.

Original languageEnglish (US)
Pages (from-to)801-805
Number of pages5
JournalJournal of Microbiological Methods
Issue number3
StatePublished - Dec 2003



  • 3-Hydroxyphenylacetylene
  • Activity
  • Chromogenic
  • Fluorogenic
  • Phenylacetylene
  • Toluene

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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