Two-dimensional gel electrophoresis as a taxonomic tool

Evidence from the centrospermae

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Two-dimensional polyacrylamide gel (2-D PAGE) electrophoresis was appraised as an experimental technique for assessing systematic relationships among higher plants and to determine at which level in the taxonomic hierarchy this technique is most generally applicable. 2-D PAGE was performed on denatured extracts of mature leaves from 25 species representing five families of the order Centrospermae (Caryophyllales, Chenopodiales) in the Angiospermae as well as Welwitschia mirabilis (Gymnospermae). Cluster analysis of a 256 spot binary-coded data set derived from the computer-encoded spot patterns of the 25 species successfully separated taxa from the individual to the familial levels of the taxonomic hierarchy in accordance with traditional taxonomic delineations of the taxa.

Original languageEnglish (US)
Pages (from-to)573-582
Number of pages10
JournalBiochemical Systematics and Ecology
Volume17
Issue number7-8
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Mirabilis
Angiosperms
two-dimensional gel electrophoresis
Electrophoresis, Gel, Two-Dimensional
Cluster analysis
Electrophoresis
polyacrylamide gel electrophoresis
Cluster Analysis
electrokinesis
Welwitschia mirabilis
gel
Gels
Caryophyllales
polyacrylamide
electrophoresis
cluster analysis
Angiospermae
gels
extracts
methodology

Keywords

  • Caryophyllales
  • Centrospermae
  • Chenopodiales
  • cluster analysis
  • phenetics
  • systematics
  • two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

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title = "Two-dimensional gel electrophoresis as a taxonomic tool: Evidence from the centrospermae",
abstract = "Two-dimensional polyacrylamide gel (2-D PAGE) electrophoresis was appraised as an experimental technique for assessing systematic relationships among higher plants and to determine at which level in the taxonomic hierarchy this technique is most generally applicable. 2-D PAGE was performed on denatured extracts of mature leaves from 25 species representing five families of the order Centrospermae (Caryophyllales, Chenopodiales) in the Angiospermae as well as Welwitschia mirabilis (Gymnospermae). Cluster analysis of a 256 spot binary-coded data set derived from the computer-encoded spot patterns of the 25 species successfully separated taxa from the individual to the familial levels of the taxonomic hierarchy in accordance with traditional taxonomic delineations of the taxa.",
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AB - Two-dimensional polyacrylamide gel (2-D PAGE) electrophoresis was appraised as an experimental technique for assessing systematic relationships among higher plants and to determine at which level in the taxonomic hierarchy this technique is most generally applicable. 2-D PAGE was performed on denatured extracts of mature leaves from 25 species representing five families of the order Centrospermae (Caryophyllales, Chenopodiales) in the Angiospermae as well as Welwitschia mirabilis (Gymnospermae). Cluster analysis of a 256 spot binary-coded data set derived from the computer-encoded spot patterns of the 25 species successfully separated taxa from the individual to the familial levels of the taxonomic hierarchy in accordance with traditional taxonomic delineations of the taxa.

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KW - Chenopodiales

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KW - phenetics

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