Tree genotype and genetically based growth traits structure twig endophyte communities

Louis J. Lamit, Matthew K. Lau, Christopher M. Sthultz, Stuart C. Wooley, Thomas G Whitham, Catherine A Gehring

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Premise of the study: Fungal endophytes asymptomatically inhabit plant tissues where they have mutualistic, parasitic, or commensal relationships with their hosts. Although plant-fungal interactions at the genotype scale have broad ecological and evolutionary implications, the sensitivity of endophytes in woody tissues to differences among plant genotypes is poorly understood. We hypothesize that (1) endophyte communities in Populus angustifolia (Salicaceae) twigs vary among tree genotypes, (2) endophyte variation is linked to quantitative tree traits, and (3) tree genotype infl uences interspecifi c fungal interactions. Methods: Endophytes were isolated from twigs of replicated P. angustifolia genotypes in a common garden and characterized with PCR-RFLP and DNA sequencing. Twig length and diameter, aboveground tree biomass, and condensed tannins were also quantifi ed. Key results: (1) Aspects of fungal community structure, including composition and total isolation frequency (i.e., abundance), varied among genotypes. (2) Aboveground biomass and twig diameter were positively associated with isolation frequency and covaried with composition, whereas twig length and condensed tannin concentration were not signifi cantly correlated to endophytes. (3) Fungal co-occurrence patterns suggested negative species interactions, but the presence of signifi cant co-occurrences was genotype dependent. Conclusions: The species is often assumed to be the most important ecological unit; however, these results indicate that genetically based trait variation within a species can infl uence an important community of associated organisms. Given the dominance of plants as primary producers and the ubiquity of endophytes, the effect of host genetic variation on endophytes has fundamental implications for our understanding of terrestrial ecosystems.

Original languageEnglish (US)
Pages (from-to)467-478
Number of pages12
JournalAmerican Journal of Botany
Volume101
Issue number3
DOIs
StatePublished - Mar 2014

Fingerprint

Endophytes
endophyte
growth traits
endophytes
genotype
Genotype
Growth
Populus angustifolia
Proanthocyanidins
tannin
proanthocyanidins
Biomass
Salicaceae
Fungal Structures
Populus
commensal
fungal communities
aboveground biomass
DNA Sequence Analysis
terrestrial ecosystem

Keywords

  • Aboveground biomass
  • Co-occurrence analysis
  • Condensed tannins
  • Endophyte
  • Populus angustifolia
  • Salicaceae
  • Tree genotype

ASJC Scopus subject areas

  • Plant Science
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Tree genotype and genetically based growth traits structure twig endophyte communities. / Lamit, Louis J.; Lau, Matthew K.; Sthultz, Christopher M.; Wooley, Stuart C.; Whitham, Thomas G; Gehring, Catherine A.

In: American Journal of Botany, Vol. 101, No. 3, 03.2014, p. 467-478.

Research output: Contribution to journalArticle

Lamit, Louis J. ; Lau, Matthew K. ; Sthultz, Christopher M. ; Wooley, Stuart C. ; Whitham, Thomas G ; Gehring, Catherine A. / Tree genotype and genetically based growth traits structure twig endophyte communities. In: American Journal of Botany. 2014 ; Vol. 101, No. 3. pp. 467-478.
@article{a37212e372ea4a6f9a17cfb711ceab96,
title = "Tree genotype and genetically based growth traits structure twig endophyte communities",
abstract = "Premise of the study: Fungal endophytes asymptomatically inhabit plant tissues where they have mutualistic, parasitic, or commensal relationships with their hosts. Although plant-fungal interactions at the genotype scale have broad ecological and evolutionary implications, the sensitivity of endophytes in woody tissues to differences among plant genotypes is poorly understood. We hypothesize that (1) endophyte communities in Populus angustifolia (Salicaceae) twigs vary among tree genotypes, (2) endophyte variation is linked to quantitative tree traits, and (3) tree genotype infl uences interspecifi c fungal interactions. Methods: Endophytes were isolated from twigs of replicated P. angustifolia genotypes in a common garden and characterized with PCR-RFLP and DNA sequencing. Twig length and diameter, aboveground tree biomass, and condensed tannins were also quantifi ed. Key results: (1) Aspects of fungal community structure, including composition and total isolation frequency (i.e., abundance), varied among genotypes. (2) Aboveground biomass and twig diameter were positively associated with isolation frequency and covaried with composition, whereas twig length and condensed tannin concentration were not signifi cantly correlated to endophytes. (3) Fungal co-occurrence patterns suggested negative species interactions, but the presence of signifi cant co-occurrences was genotype dependent. Conclusions: The species is often assumed to be the most important ecological unit; however, these results indicate that genetically based trait variation within a species can infl uence an important community of associated organisms. Given the dominance of plants as primary producers and the ubiquity of endophytes, the effect of host genetic variation on endophytes has fundamental implications for our understanding of terrestrial ecosystems.",
keywords = "Aboveground biomass, Co-occurrence analysis, Condensed tannins, Endophyte, Populus angustifolia, Salicaceae, Tree genotype",
author = "Lamit, {Louis J.} and Lau, {Matthew K.} and Sthultz, {Christopher M.} and Wooley, {Stuart C.} and Whitham, {Thomas G} and Gehring, {Catherine A}",
year = "2014",
month = "3",
doi = "10.3732/ajb.1400034",
language = "English (US)",
volume = "101",
pages = "467--478",
journal = "American Journal of Botany",
issn = "0002-9122",
publisher = "Botanical Society of America Inc.",
number = "3",

}

TY - JOUR

T1 - Tree genotype and genetically based growth traits structure twig endophyte communities

AU - Lamit, Louis J.

AU - Lau, Matthew K.

AU - Sthultz, Christopher M.

AU - Wooley, Stuart C.

AU - Whitham, Thomas G

AU - Gehring, Catherine A

PY - 2014/3

Y1 - 2014/3

N2 - Premise of the study: Fungal endophytes asymptomatically inhabit plant tissues where they have mutualistic, parasitic, or commensal relationships with their hosts. Although plant-fungal interactions at the genotype scale have broad ecological and evolutionary implications, the sensitivity of endophytes in woody tissues to differences among plant genotypes is poorly understood. We hypothesize that (1) endophyte communities in Populus angustifolia (Salicaceae) twigs vary among tree genotypes, (2) endophyte variation is linked to quantitative tree traits, and (3) tree genotype infl uences interspecifi c fungal interactions. Methods: Endophytes were isolated from twigs of replicated P. angustifolia genotypes in a common garden and characterized with PCR-RFLP and DNA sequencing. Twig length and diameter, aboveground tree biomass, and condensed tannins were also quantifi ed. Key results: (1) Aspects of fungal community structure, including composition and total isolation frequency (i.e., abundance), varied among genotypes. (2) Aboveground biomass and twig diameter were positively associated with isolation frequency and covaried with composition, whereas twig length and condensed tannin concentration were not signifi cantly correlated to endophytes. (3) Fungal co-occurrence patterns suggested negative species interactions, but the presence of signifi cant co-occurrences was genotype dependent. Conclusions: The species is often assumed to be the most important ecological unit; however, these results indicate that genetically based trait variation within a species can infl uence an important community of associated organisms. Given the dominance of plants as primary producers and the ubiquity of endophytes, the effect of host genetic variation on endophytes has fundamental implications for our understanding of terrestrial ecosystems.

AB - Premise of the study: Fungal endophytes asymptomatically inhabit plant tissues where they have mutualistic, parasitic, or commensal relationships with their hosts. Although plant-fungal interactions at the genotype scale have broad ecological and evolutionary implications, the sensitivity of endophytes in woody tissues to differences among plant genotypes is poorly understood. We hypothesize that (1) endophyte communities in Populus angustifolia (Salicaceae) twigs vary among tree genotypes, (2) endophyte variation is linked to quantitative tree traits, and (3) tree genotype infl uences interspecifi c fungal interactions. Methods: Endophytes were isolated from twigs of replicated P. angustifolia genotypes in a common garden and characterized with PCR-RFLP and DNA sequencing. Twig length and diameter, aboveground tree biomass, and condensed tannins were also quantifi ed. Key results: (1) Aspects of fungal community structure, including composition and total isolation frequency (i.e., abundance), varied among genotypes. (2) Aboveground biomass and twig diameter were positively associated with isolation frequency and covaried with composition, whereas twig length and condensed tannin concentration were not signifi cantly correlated to endophytes. (3) Fungal co-occurrence patterns suggested negative species interactions, but the presence of signifi cant co-occurrences was genotype dependent. Conclusions: The species is often assumed to be the most important ecological unit; however, these results indicate that genetically based trait variation within a species can infl uence an important community of associated organisms. Given the dominance of plants as primary producers and the ubiquity of endophytes, the effect of host genetic variation on endophytes has fundamental implications for our understanding of terrestrial ecosystems.

KW - Aboveground biomass

KW - Co-occurrence analysis

KW - Condensed tannins

KW - Endophyte

KW - Populus angustifolia

KW - Salicaceae

KW - Tree genotype

UR - http://www.scopus.com/inward/record.url?scp=84897881627&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897881627&partnerID=8YFLogxK

U2 - 10.3732/ajb.1400034

DO - 10.3732/ajb.1400034

M3 - Article

VL - 101

SP - 467

EP - 478

JO - American Journal of Botany

JF - American Journal of Botany

SN - 0002-9122

IS - 3

ER -