Abstract
14-3-3 Proteins are expressed in most eukaryotes organisms and play varied and crucial roles in a wide range of regulatory processes. In mammalian cells, seven 14-3-3 isoforms have been identified. However, it is not known what effect infection has on 14-3-3 isoform expression. In this study human colonic carcinoma cell lines were infected with Toxoplasma gondii for 24 h and expression of 14-3-3 proteins was determined by RT-PCR. HT-29 cells only expressed 3 out of the 7 isoforms while 5 and all 7 isoforms were found in HCT-116 and Caco-2 cells, respectively. Infection had little or no effect in the expression of 14-3-3γ, ε, σ, and ξ; but in HCT-116 cells induced expression of 14-3-3η and σ, while 14-3-3β, η, and ξ were induced in HT-29 cells. If 14-3-3 proteins are involved in cell survival and/or prevention of parasite replication, longer incubation times may be required as no differences in percentage of infection were found among the cell lines at 24 h post-infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 134-138 |
| Number of pages | 5 |
| Journal | Experimental Parasitology |
| Volume | 118 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2008 |
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Keywords
- 14-3-3 Isoforms
- Colon cancer cells
- Colonic epithelial cells
- RT-PCR
- Toxoplasma
ASJC Scopus subject areas
- Immunology
- Parasitology
- Infectious Diseases
Cite this
Toxoplasma gondii : Effect of infection on expression of 14-3-3 proteins in human epithelial cells. / Monroy, Fernando P.
In: Experimental Parasitology, Vol. 118, No. 1, 01.2008, p. 134-138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Toxoplasma gondii
T2 - Effect of infection on expression of 14-3-3 proteins in human epithelial cells
AU - Monroy, Fernando P
PY - 2008/1
Y1 - 2008/1
N2 - 14-3-3 Proteins are expressed in most eukaryotes organisms and play varied and crucial roles in a wide range of regulatory processes. In mammalian cells, seven 14-3-3 isoforms have been identified. However, it is not known what effect infection has on 14-3-3 isoform expression. In this study human colonic carcinoma cell lines were infected with Toxoplasma gondii for 24 h and expression of 14-3-3 proteins was determined by RT-PCR. HT-29 cells only expressed 3 out of the 7 isoforms while 5 and all 7 isoforms were found in HCT-116 and Caco-2 cells, respectively. Infection had little or no effect in the expression of 14-3-3γ, ε, σ, and ξ; but in HCT-116 cells induced expression of 14-3-3η and σ, while 14-3-3β, η, and ξ were induced in HT-29 cells. If 14-3-3 proteins are involved in cell survival and/or prevention of parasite replication, longer incubation times may be required as no differences in percentage of infection were found among the cell lines at 24 h post-infection.
AB - 14-3-3 Proteins are expressed in most eukaryotes organisms and play varied and crucial roles in a wide range of regulatory processes. In mammalian cells, seven 14-3-3 isoforms have been identified. However, it is not known what effect infection has on 14-3-3 isoform expression. In this study human colonic carcinoma cell lines were infected with Toxoplasma gondii for 24 h and expression of 14-3-3 proteins was determined by RT-PCR. HT-29 cells only expressed 3 out of the 7 isoforms while 5 and all 7 isoforms were found in HCT-116 and Caco-2 cells, respectively. Infection had little or no effect in the expression of 14-3-3γ, ε, σ, and ξ; but in HCT-116 cells induced expression of 14-3-3η and σ, while 14-3-3β, η, and ξ were induced in HT-29 cells. If 14-3-3 proteins are involved in cell survival and/or prevention of parasite replication, longer incubation times may be required as no differences in percentage of infection were found among the cell lines at 24 h post-infection.
KW - 14-3-3 Isoforms
KW - Colon cancer cells
KW - Colonic epithelial cells
KW - RT-PCR
KW - Toxoplasma
UR - http://www.scopus.com/inward/record.url?scp=37349117650&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37349117650&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2007.07.008
DO - 10.1016/j.exppara.2007.07.008
M3 - Article
C2 - 17825295
AN - SCOPUS:37349117650
VL - 118
SP - 134
EP - 138
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 1
ER -