Towards a rapid molecular diagnostic for melioidosis

Comparison of DNA extraction methods from clinical specimens

Leisha J. Richardson, Mirjam Kaestli, Mark Mayo, Jolene R. Bowers, Apichai Tuanyok, Jim Schupp, David Engelthaler, David M Wagner, Paul S Keim, Bart J. Currie

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

Original languageEnglish (US)
Pages (from-to)179-181
Number of pages3
JournalJournal of Microbiological Methods
Volume88
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Melioidosis
Molecular Pathology
DNA
Blood Buffy Coat
Burkholderia pseudomallei
Sputum
Real-Time Polymerase Chain Reaction
Urine

Keywords

  • Burkholderia pseudomallei
  • Clinical specimen
  • Diagnostic
  • DNA extraction
  • Melioidosis
  • Real-time PCR

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Richardson, L. J., Kaestli, M., Mayo, M., Bowers, J. R., Tuanyok, A., Schupp, J., ... Currie, B. J. (2012). Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens. Journal of Microbiological Methods, 88(1), 179-181. https://doi.org/10.1016/j.mimet.2011.10.023

Towards a rapid molecular diagnostic for melioidosis : Comparison of DNA extraction methods from clinical specimens. / Richardson, Leisha J.; Kaestli, Mirjam; Mayo, Mark; Bowers, Jolene R.; Tuanyok, Apichai; Schupp, Jim; Engelthaler, David; Wagner, David M; Keim, Paul S; Currie, Bart J.

In: Journal of Microbiological Methods, Vol. 88, No. 1, 01.2012, p. 179-181.

Research output: Contribution to journalArticle

Richardson, Leisha J. ; Kaestli, Mirjam ; Mayo, Mark ; Bowers, Jolene R. ; Tuanyok, Apichai ; Schupp, Jim ; Engelthaler, David ; Wagner, David M ; Keim, Paul S ; Currie, Bart J. / Towards a rapid molecular diagnostic for melioidosis : Comparison of DNA extraction methods from clinical specimens. In: Journal of Microbiological Methods. 2012 ; Vol. 88, No. 1. pp. 179-181.
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