Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

Leisha J. Richardson, Mirjam Kaestli, Mark Mayo, Jolene R. Bowers, Apichai Tuanyok, Jim Schupp, David Engelthaler, David M. Wagner, Paul S. Keim, Bart J. Currie

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

Original languageEnglish (US)
Pages (from-to)179-181
Number of pages3
JournalJournal of Microbiological Methods
Volume88
Issue number1
DOIs
StatePublished - Jan 1 2012

Keywords

  • Burkholderia pseudomallei
  • Clinical specimen
  • DNA extraction
  • Diagnostic
  • Melioidosis
  • Real-time PCR

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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