Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

Leisha J. Richardson, Mirjam Kaestli, Mark Mayo, Jolene R. Bowers, Apichai Tuanyok, Jim Schupp, David Engelthaler, David M Wagner, Paul S Keim, Bart J. Currie

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

Original languageEnglish (US)
Pages (from-to)179-181
Number of pages3
JournalJournal of Microbiological Methods
Volume88
Issue number1
DOIs
StatePublished - Jan 2012

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Keywords

  • Burkholderia pseudomallei
  • Clinical specimen
  • Diagnostic
  • DNA extraction
  • Melioidosis
  • Real-time PCR

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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