The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems

Kiel Nikolakakis, Saba Amber, J. Scott Wilbur, Elie J. Diner, Stephanie K. Aoki, Stephen J. Poole, Apichai Tuanyok, Paul S Keim, Sharon Peacock, Christopher S. Hayes, David A. Low

Research output: Contribution to journalArticle

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Abstract

Burkholderia pseudomallei is a category B pathogen and the causative agent of melioidosis - a serious infectious disease that is typically acquired directly from environmental reservoirs. Nearly all B. pseudomallei strains sequenced to date (>85 isolates) contain gene clusters that are related to the contact-dependent growth inhibition (CDI) systems of γ-proteobacteria. CDI systems from Escherichia coli and Dickeya dadantii play significant roles in bacterial competition, suggesting these systems may also contribute to the competitive fitness of B. pseudomallei. Here, we identify 10 distinct CDI systems in B. pseudomallei based on polymorphisms within the cdiA-CT/cdiI coding regions, which are predicted to encode CdiA-CT/CdiI toxin/immunity protein pairs. Biochemical analysis of three B. pseudomallei CdiA-CTs revealed that each protein possesses a distinct tRNase activity capable of inhibiting cell growth. These toxin activities are blocked by cognate CdiI immunity proteins, which specifically bind the CdiA-CT and protect cells from growth inhibition. Using Burkholderia thailandensis E264 as a model, we show that a CDI system from B. pseudomallei 1026b mediates CDI and is capable of delivering CdiA-CT toxins derived from other B. pseudomallei strains. These results demonstrate that Burkholderia species contain functional CDI systems, which may confer a competitive advantage to these bacteria.

Original languageEnglish (US)
Pages (from-to)516-529
Number of pages14
JournalMolecular Microbiology
Volume84
Issue number3
DOIs
StatePublished - May 2012

Fingerprint

Burkholderia pseudomallei
Immunity
Growth
Burkholderia
Melioidosis
Proteobacteria
Proteins
Multigene Family
Communicable Diseases
Escherichia coli
Bacteria

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Nikolakakis, K., Amber, S., Wilbur, J. S., Diner, E. J., Aoki, S. K., Poole, S. J., ... Low, D. A. (2012). The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems. Molecular Microbiology, 84(3), 516-529. https://doi.org/10.1111/j.1365-2958.2012.08039.x

The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems. / Nikolakakis, Kiel; Amber, Saba; Wilbur, J. Scott; Diner, Elie J.; Aoki, Stephanie K.; Poole, Stephen J.; Tuanyok, Apichai; Keim, Paul S; Peacock, Sharon; Hayes, Christopher S.; Low, David A.

In: Molecular Microbiology, Vol. 84, No. 3, 05.2012, p. 516-529.

Research output: Contribution to journalArticle

Nikolakakis, K, Amber, S, Wilbur, JS, Diner, EJ, Aoki, SK, Poole, SJ, Tuanyok, A, Keim, PS, Peacock, S, Hayes, CS & Low, DA 2012, 'The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems', Molecular Microbiology, vol. 84, no. 3, pp. 516-529. https://doi.org/10.1111/j.1365-2958.2012.08039.x
Nikolakakis, Kiel ; Amber, Saba ; Wilbur, J. Scott ; Diner, Elie J. ; Aoki, Stephanie K. ; Poole, Stephen J. ; Tuanyok, Apichai ; Keim, Paul S ; Peacock, Sharon ; Hayes, Christopher S. ; Low, David A. / The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems. In: Molecular Microbiology. 2012 ; Vol. 84, No. 3. pp. 516-529.
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