The role of curcumin in modulating colonic microbiota during colitis and colon cancer prevention

Rita Marie T Mcfadden, Claire B. Larmonier, Kareem W. Shehab, Monica Midura-Kiela, Rajalakshmy Ramalingam, Christy A. Harrison, David G. Besselsen, John H. Chase, James G Caporaso, Christian Jobin, Fayez K. Ghishan, Pawel R. Kiela

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: Intestinal microbiota influences the progression of colitis-associated colorectal cancer. With diet being a key determinant of the gut microbial ecology, dietary interventions are an attractive avenue for the prevention of colitis-associated colorectal cancer. Curcumin is the most active constituent of the ground rhizome of the Curcuma longa plant, which has been demonstrated to have anti-inflammatory, antioxidative, and antiproliferative properties. Methods: Il10 -/- mice on 129/SvEv background were used as a model of colitis-associated colorectal cancer. Starting at 10 weeks of age, wild-type or Il10 -/- mice received 6 weekly intraperitoneal injections of azoxymethane (AOM) or phosphate-buffered saline (PBS) and were started on either a control or a curcumin-supplemented diet. Stools were collected every 4 weeks for microbial community analysis. Mice were killed at 30 weeks of age. Results: Curcumin-supplemented diet increased survival, decreased colon weight/length ratio, and, at 0.5%, entirely eliminated tumor burden. Although colonic histology indicated improvement with curcumin, no effects of mucosal immune responses have been observed in PBS/Il10 -/- mice and limited effects were seen in AOM/Il10 -/- mice. In wild-type and in Il10 -/- mice, curcumin increased bacterial richness, prevented age-related decrease in alpha diversity, increased the relative abundance of Lactobacillales, and decreased Coriobacterales order. Taxonomic profile of AOM/Il10 -/- mice receiving curcumin was more similar to those of wild-type mice than those fed control diet. Conclusions: In AOM/Il10 -/- model, curcumin reduced or eliminated colonic tumor burden with limited effects on mucosal immune responses. The beneficial effect of curcumin on tumorigenesis was associated with the maintenance of a more diverse colonic microbial ecology.

Original languageEnglish (US)
Pages (from-to)2483-2494
Number of pages12
JournalInflammatory Bowel Diseases
Volume21
Issue number11
DOIs
StatePublished - Jul 25 2015

Fingerprint

Curcumin
Microbiota
Colitis
Colonic Neoplasms
Interleukin-10
Azoxymethane
Diet
Colorectal Neoplasms
Mucosal Immunity
Ecology
Tumor Burden
Lactobacillales
Phosphates
129 Strain Mouse
Curcuma
Rhizome
Intraperitoneal Injections
Histology
Colon
Carcinogenesis

Keywords

  • 16S
  • colitis-associated colon cancer
  • curcumin
  • diet
  • microbiota
  • ribosomal RNA

ASJC Scopus subject areas

  • Gastroenterology
  • Immunology and Allergy

Cite this

Mcfadden, R. M. T., Larmonier, C. B., Shehab, K. W., Midura-Kiela, M., Ramalingam, R., Harrison, C. A., ... Kiela, P. R. (2015). The role of curcumin in modulating colonic microbiota during colitis and colon cancer prevention. Inflammatory Bowel Diseases, 21(11), 2483-2494. https://doi.org/10.1097/MIB.0000000000000522

The role of curcumin in modulating colonic microbiota during colitis and colon cancer prevention. / Mcfadden, Rita Marie T; Larmonier, Claire B.; Shehab, Kareem W.; Midura-Kiela, Monica; Ramalingam, Rajalakshmy; Harrison, Christy A.; Besselsen, David G.; Chase, John H.; Caporaso, James G; Jobin, Christian; Ghishan, Fayez K.; Kiela, Pawel R.

In: Inflammatory Bowel Diseases, Vol. 21, No. 11, 25.07.2015, p. 2483-2494.

Research output: Contribution to journalArticle

Mcfadden, RMT, Larmonier, CB, Shehab, KW, Midura-Kiela, M, Ramalingam, R, Harrison, CA, Besselsen, DG, Chase, JH, Caporaso, JG, Jobin, C, Ghishan, FK & Kiela, PR 2015, 'The role of curcumin in modulating colonic microbiota during colitis and colon cancer prevention', Inflammatory Bowel Diseases, vol. 21, no. 11, pp. 2483-2494. https://doi.org/10.1097/MIB.0000000000000522
Mcfadden RMT, Larmonier CB, Shehab KW, Midura-Kiela M, Ramalingam R, Harrison CA et al. The role of curcumin in modulating colonic microbiota during colitis and colon cancer prevention. Inflammatory Bowel Diseases. 2015 Jul 25;21(11):2483-2494. https://doi.org/10.1097/MIB.0000000000000522
Mcfadden, Rita Marie T ; Larmonier, Claire B. ; Shehab, Kareem W. ; Midura-Kiela, Monica ; Ramalingam, Rajalakshmy ; Harrison, Christy A. ; Besselsen, David G. ; Chase, John H. ; Caporaso, James G ; Jobin, Christian ; Ghishan, Fayez K. ; Kiela, Pawel R. / The role of curcumin in modulating colonic microbiota during colitis and colon cancer prevention. In: Inflammatory Bowel Diseases. 2015 ; Vol. 21, No. 11. pp. 2483-2494.
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AU - Ramalingam, Rajalakshmy

AU - Harrison, Christy A.

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