The otologic microbiome

A study of the bacterial microbiota in a pediatric patient with chronic serous otitis media using 16SrRNA gene-based pyroseg

Cindy M. Liu, Maura K. Cosetti, Maliha Aziz, Jordan L. Buchhagen, Tania L. Contente-Cuomo, Lance B. Price, Paul S Keim, Anil K. Lalwani

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objective: To characterize bacterial microbiota in middle ear, adenoid, and tonsil specimens using 16SrRNA gene-based pyrosequencing analysis. Design: Cross-sectional study of bacterial microbiota in middle ear, adenoid, and tonsil specimens from a pediatric patient with chronic serous otitis media. Middle ear, adenoid, and tonsil specimens from a pediatric patient were collected and underwent cell lysis and DNA isolation. Pyrosequencing was performed on the 454 Life Sciences GS FLX platform (Roche Diagnostics Corp, Branford, Connecticut). Pyrosequencing data were processed, quality-checked, and taxonomically classified to generate an abundance-based matrix. Ecological analyses were performed. Setting: Academic, tertiary referral center. Main Outcome Measures: Comparative microbiome analysis. Results: We detected a total of 17 unique bacterial families, with 9, 9, and 12 bacterial families from the middle ear, tonsil, and adenoid specimens, respectively. Pseudomonadaceae dominated the middle ear microbiota at 82.7% relative abundance, whereas Streptococcaceae dominated the tonsil microbiota at 69.2%. Multiple bacteria, including Pseudomonadaceae, Streptococcaceae, Fusobacteriaceae, and Pasteurellaceae, dominated the adenoid microbiota. Overlap between the middle ear and the tonsil microbiota was minimal. In contrast, the adenoid microbiota encompassed bacteria detected from middle ear and tonsil. Conclusions: Bacterial community analysis using pyrosequencing analysis revealed diverse, previously unknown bacterial communities in a set of pediatric middle ear, tonsil, and adenoid specimens. Our findings suggest that the adenoid may be a source site for both the middle ear and tonsil microbiota. An ecological framework is appropriate in comparative analysis of microbiota from nonsterile body sites.

Original languageEnglish (US)
Pages (from-to)664-668
Number of pages5
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume137
Issue number7
DOIs
StatePublished - Jul 2011

Fingerprint

Otitis Media with Effusion
Microbiota
Adenoids
Palatine Tonsil
Middle Ear
Pediatrics
Genes
Streptococcaceae
Pseudomonadaceae
Pasteurellaceae
Bacteria
Biological Science Disciplines
Tertiary Care Centers
Cross-Sectional Studies
Outcome Assessment (Health Care)

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery

Cite this

The otologic microbiome : A study of the bacterial microbiota in a pediatric patient with chronic serous otitis media using 16SrRNA gene-based pyroseg. / Liu, Cindy M.; Cosetti, Maura K.; Aziz, Maliha; Buchhagen, Jordan L.; Contente-Cuomo, Tania L.; Price, Lance B.; Keim, Paul S; Lalwani, Anil K.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 137, No. 7, 07.2011, p. 664-668.

Research output: Contribution to journalArticle

Liu, Cindy M. ; Cosetti, Maura K. ; Aziz, Maliha ; Buchhagen, Jordan L. ; Contente-Cuomo, Tania L. ; Price, Lance B. ; Keim, Paul S ; Lalwani, Anil K. / The otologic microbiome : A study of the bacterial microbiota in a pediatric patient with chronic serous otitis media using 16SrRNA gene-based pyroseg. In: Archives of Otolaryngology - Head and Neck Surgery. 2011 ; Vol. 137, No. 7. pp. 664-668.
@article{0abcfa7e076c4aeca56f45161fc30980,
title = "The otologic microbiome: A study of the bacterial microbiota in a pediatric patient with chronic serous otitis media using 16SrRNA gene-based pyroseg",
abstract = "Objective: To characterize bacterial microbiota in middle ear, adenoid, and tonsil specimens using 16SrRNA gene-based pyrosequencing analysis. Design: Cross-sectional study of bacterial microbiota in middle ear, adenoid, and tonsil specimens from a pediatric patient with chronic serous otitis media. Middle ear, adenoid, and tonsil specimens from a pediatric patient were collected and underwent cell lysis and DNA isolation. Pyrosequencing was performed on the 454 Life Sciences GS FLX platform (Roche Diagnostics Corp, Branford, Connecticut). Pyrosequencing data were processed, quality-checked, and taxonomically classified to generate an abundance-based matrix. Ecological analyses were performed. Setting: Academic, tertiary referral center. Main Outcome Measures: Comparative microbiome analysis. Results: We detected a total of 17 unique bacterial families, with 9, 9, and 12 bacterial families from the middle ear, tonsil, and adenoid specimens, respectively. Pseudomonadaceae dominated the middle ear microbiota at 82.7{\%} relative abundance, whereas Streptococcaceae dominated the tonsil microbiota at 69.2{\%}. Multiple bacteria, including Pseudomonadaceae, Streptococcaceae, Fusobacteriaceae, and Pasteurellaceae, dominated the adenoid microbiota. Overlap between the middle ear and the tonsil microbiota was minimal. In contrast, the adenoid microbiota encompassed bacteria detected from middle ear and tonsil. Conclusions: Bacterial community analysis using pyrosequencing analysis revealed diverse, previously unknown bacterial communities in a set of pediatric middle ear, tonsil, and adenoid specimens. Our findings suggest that the adenoid may be a source site for both the middle ear and tonsil microbiota. An ecological framework is appropriate in comparative analysis of microbiota from nonsterile body sites.",
author = "Liu, {Cindy M.} and Cosetti, {Maura K.} and Maliha Aziz and Buchhagen, {Jordan L.} and Contente-Cuomo, {Tania L.} and Price, {Lance B.} and Keim, {Paul S} and Lalwani, {Anil K.}",
year = "2011",
month = "7",
doi = "10.1001/archoto.2011.116",
language = "English (US)",
volume = "137",
pages = "664--668",
journal = "JAMA Otolaryngology - Head and Neck Surgery",
issn = "2168-6181",
publisher = "American Medical Association",
number = "7",

}

TY - JOUR

T1 - The otologic microbiome

T2 - A study of the bacterial microbiota in a pediatric patient with chronic serous otitis media using 16SrRNA gene-based pyroseg

AU - Liu, Cindy M.

AU - Cosetti, Maura K.

AU - Aziz, Maliha

AU - Buchhagen, Jordan L.

AU - Contente-Cuomo, Tania L.

AU - Price, Lance B.

AU - Keim, Paul S

AU - Lalwani, Anil K.

PY - 2011/7

Y1 - 2011/7

N2 - Objective: To characterize bacterial microbiota in middle ear, adenoid, and tonsil specimens using 16SrRNA gene-based pyrosequencing analysis. Design: Cross-sectional study of bacterial microbiota in middle ear, adenoid, and tonsil specimens from a pediatric patient with chronic serous otitis media. Middle ear, adenoid, and tonsil specimens from a pediatric patient were collected and underwent cell lysis and DNA isolation. Pyrosequencing was performed on the 454 Life Sciences GS FLX platform (Roche Diagnostics Corp, Branford, Connecticut). Pyrosequencing data were processed, quality-checked, and taxonomically classified to generate an abundance-based matrix. Ecological analyses were performed. Setting: Academic, tertiary referral center. Main Outcome Measures: Comparative microbiome analysis. Results: We detected a total of 17 unique bacterial families, with 9, 9, and 12 bacterial families from the middle ear, tonsil, and adenoid specimens, respectively. Pseudomonadaceae dominated the middle ear microbiota at 82.7% relative abundance, whereas Streptococcaceae dominated the tonsil microbiota at 69.2%. Multiple bacteria, including Pseudomonadaceae, Streptococcaceae, Fusobacteriaceae, and Pasteurellaceae, dominated the adenoid microbiota. Overlap between the middle ear and the tonsil microbiota was minimal. In contrast, the adenoid microbiota encompassed bacteria detected from middle ear and tonsil. Conclusions: Bacterial community analysis using pyrosequencing analysis revealed diverse, previously unknown bacterial communities in a set of pediatric middle ear, tonsil, and adenoid specimens. Our findings suggest that the adenoid may be a source site for both the middle ear and tonsil microbiota. An ecological framework is appropriate in comparative analysis of microbiota from nonsterile body sites.

AB - Objective: To characterize bacterial microbiota in middle ear, adenoid, and tonsil specimens using 16SrRNA gene-based pyrosequencing analysis. Design: Cross-sectional study of bacterial microbiota in middle ear, adenoid, and tonsil specimens from a pediatric patient with chronic serous otitis media. Middle ear, adenoid, and tonsil specimens from a pediatric patient were collected and underwent cell lysis and DNA isolation. Pyrosequencing was performed on the 454 Life Sciences GS FLX platform (Roche Diagnostics Corp, Branford, Connecticut). Pyrosequencing data were processed, quality-checked, and taxonomically classified to generate an abundance-based matrix. Ecological analyses were performed. Setting: Academic, tertiary referral center. Main Outcome Measures: Comparative microbiome analysis. Results: We detected a total of 17 unique bacterial families, with 9, 9, and 12 bacterial families from the middle ear, tonsil, and adenoid specimens, respectively. Pseudomonadaceae dominated the middle ear microbiota at 82.7% relative abundance, whereas Streptococcaceae dominated the tonsil microbiota at 69.2%. Multiple bacteria, including Pseudomonadaceae, Streptococcaceae, Fusobacteriaceae, and Pasteurellaceae, dominated the adenoid microbiota. Overlap between the middle ear and the tonsil microbiota was minimal. In contrast, the adenoid microbiota encompassed bacteria detected from middle ear and tonsil. Conclusions: Bacterial community analysis using pyrosequencing analysis revealed diverse, previously unknown bacterial communities in a set of pediatric middle ear, tonsil, and adenoid specimens. Our findings suggest that the adenoid may be a source site for both the middle ear and tonsil microbiota. An ecological framework is appropriate in comparative analysis of microbiota from nonsterile body sites.

UR - http://www.scopus.com/inward/record.url?scp=79960491617&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960491617&partnerID=8YFLogxK

U2 - 10.1001/archoto.2011.116

DO - 10.1001/archoto.2011.116

M3 - Article

VL - 137

SP - 664

EP - 668

JO - JAMA Otolaryngology - Head and Neck Surgery

JF - JAMA Otolaryngology - Head and Neck Surgery

SN - 2168-6181

IS - 7

ER -