The missing link in petrobactin biosynthesis: asbF encodes a (-)-3-dehydroshikimate dehydratase

David T. Fox, Kinya Hotta, Chu Young Kim, Andrew T. Koppisch

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Abstract

The siderophore petrobactin harbors unique 3,4-dihydroxybenzoyl iron-liganding groups. These moieties are known to be synthesized from shikimate pathway precursors, but no reports of the biosynthetic enzymes responsible for this conversion have been published. The gene encoding AsbF from Bacillus thuringiensis 97-27 was overexpressed in an Escherichia coli host. AsbF rapidly and efficiently transforms (-)-3-dehydroshikimate (DHS) into 3,4-dihydroxybenzoate (kcatDHS = 217 ± 10 min -1; KmDHS = 125 ± 14 μM) at 37°C and has an absolute requirement for divalent metal. Finally, the pH versus kcatDHS profile revealed two ionizable groups (pK a1 = 7.9 ± 0.1, and pKa2 = 9.3 ± 0.1).

Original languageEnglish (US)
Pages (from-to)12251-12253
Number of pages3
JournalBiochemistry
Volume47
Issue number47
DOIs
StatePublished - Nov 25 2008
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

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