The international sinonasal microbiome study: A multicentre, multinational characterization of sinonasal bacterial ecology

Sathish Paramasivan, Ahmed Bassiouni, Arron Shiffer, Matthew R. Dillon, Emily K. Cope, Clare Cooksley, Mahnaz Ramezanpour, Sophia Moraitis, Mohammad Javed Ali, Benjamin Bleier, Claudio Callejas, Marjolein E. Cornet, Richard G. Douglas, Daniel Dutra, Christos Georgalas, Richard J. Harvey, Peter H. Hwang, Amber U. Luong, Rodney J. Schlosser, Pongsakorn TantilipikornMarc A. Tewfik, Sarah Vreugde, Peter John Wormald, J. Gregory Caporaso, Alkis J. Psaltis

Research output: Contribution to journalArticle

Abstract

The sinonasal microbiome remains poorly defined, with our current knowledge based on a few cohort studies whose findings are inconsistent. Furthermore, the variability of the sinus microbiome across geographical divides remains unexplored. We characterize the sinonasal microbiome and its geographical variations in both health and disease using 16S rRNA gene sequencing of 410 individuals from across the world. Although the sinus microbial ecology is highly variable between individuals, we identify a core microbiome comprised of Corynebacterium, Staphylococcus, Streptococcus, Haemophilus and Moraxella species in both healthy and chronic rhinosinusitis (CRS) cohorts. Corynebacterium (mean relative abundance = 44.02%) and Staphylococcus (mean relative abundance = 27.34%) appear particularly dominant in the majority of patients sampled. Amongst patients suffering from CRS with nasal polyps, a statistically significant reduction in relative abundance of Corynebacterium (40.29% vs 50.43%; P =.02) was identified. Despite some measured differences in microbiome composition and diversity between some of the participating centres in our cohort, these differences would not alter the general pattern of core organisms described. Nevertheless, atypical or unusual organisms reported in short-read amplicon sequencing studies and that are not part of the core microbiome should be interpreted with caution. The delineation of the sinonasal microbiome and standardized methodology described within our study will enable further characterization and translational application of the sinus microbiota.

Original languageEnglish (US)
JournalAllergy: European Journal of Allergy and Clinical Immunology
DOIs
StateAccepted/In press - Jan 1 2020

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Keywords

  • 16S rRNA gene
  • chronic rhinosinusitis
  • microbiome
  • next-generation sequencing
  • sinus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Paramasivan, S., Bassiouni, A., Shiffer, A., Dillon, M. R., Cope, E. K., Cooksley, C., Ramezanpour, M., Moraitis, S., Ali, M. J., Bleier, B., Callejas, C., Cornet, M. E., Douglas, R. G., Dutra, D., Georgalas, C., Harvey, R. J., Hwang, P. H., Luong, A. U., Schlosser, R. J., ... Psaltis, A. J. (Accepted/In press). The international sinonasal microbiome study: A multicentre, multinational characterization of sinonasal bacterial ecology. Allergy: European Journal of Allergy and Clinical Immunology. https://doi.org/10.1111/all.14276