The innate immune response may be important for surviving plague in wild Gunnison's prairie dogs

Joseph D. Busch, Roger Van Andel, Nathan E. Stone, Kacy R. Cobble, Roxanne Nottingham, Judy Lee, Michael VerSteeg, Jeff Corcoran, Jennifer Cordova, William Van Pelt, Megan M. Shuey, Jeffrey T. Foster, James M. Schupp, Stephen Beckstrom-Sternberg, James Beckstrom-Sternberg, Paul Keim, Susan Smith, Julia Rodriguez-Ramos, Judy L. Williamson, Tonie E. RockeDavid M. Wagner

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Prairie dogs (Cynomys spp.) are highly susceptible to Yersinia pestis, with $99% mortality reported from multiple studies of plague epizootics. A colony of Gunnison's prairie dogs (Cynomys gunnisoni) in the Aubrey Valley (AV) of northern Arizona appears to have survived several regional epizootics of plague, whereas nearby colonies have been severely affected by Y. pestis. To examine potential mechanisms accounting for survival in the AV colony, we conducted a laboratory Y. pestis challenge experiment on 60 wild-caught prairie dogs from AV and from a nearby, large colony with frequent past outbreaks of plague, Espee (n530 per colony). Test animals were challenged subcutaneously with the fully virulent Y. pestis strain CO92 at three doses: 50, 5,000, and 50,000 colony-forming units (cfu); this range is lethal in black-tailed prairie dogs (Cynomys ludovicianus). Contrary to our expectations, only 40% of the animals died. Although mortality trended higher in the Espee colony (50%) compared with AV (30%), the differences among infectious doses were not statistically significant. Only 39% of the survivors developed moderate to high antibody levels to Y. pestis, indicating that mechanisms other than humoral immunity are important in resistance to plague. The ratio of neutrophils to lymphocytes was not correlated with plague survival in this study. However, several immune proteins with roles in innate immunity (VCAM-1, CXCL-1, and vWF) were upregulated during plague infection and warrant further inquiry into their role for protection against this disease. These results suggest plague resistance exists in wild populations of the Gunnison's prairie dog and provide important directions for future studies.

Original languageEnglish (US)
Pages (from-to)920-931
Number of pages12
JournalJournal of wildlife diseases
Volume49
Issue number4
DOIs
StatePublished - Oct 1 2013

Keywords

  • Aubrey valley
  • Innate immunity
  • Plague resistance
  • Prairie dog
  • Yersinia pestis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

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    Busch, J. D., Van Andel, R., Stone, N. E., Cobble, K. R., Nottingham, R., Lee, J., VerSteeg, M., Corcoran, J., Cordova, J., Van Pelt, W., Shuey, M. M., Foster, J. T., Schupp, J. M., Beckstrom-Sternberg, S., Beckstrom-Sternberg, J., Keim, P., Smith, S., Rodriguez-Ramos, J., Williamson, J. L., ... Wagner, D. M. (2013). The innate immune response may be important for surviving plague in wild Gunnison's prairie dogs. Journal of wildlife diseases, 49(4), 920-931. https://doi.org/10.7589/2012-08-209