The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharide

Apichai Tuanyok, Joshua K. Stone, Mark Mayo, Mirjam Kaestli, Jeffrey Gruendike, Shalamar Georgia, Stephanie Warrington, Travis Mullins, Christopher J. Allender, David M Wagner, Narisara Chantratita, Sharon J. Peacock, Bart J. Currie, Paul S Keim

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS) is one of the most important virulence and antigenic components of Burkholderia pseudomallei, the causative agent of melioidosis. LPS diversity in B. pseudomallei has been described as typical, atypical or rough, based upon banding patterns on SDS-PAGE. Here, we studied the genetic and molecular basis of these phenotypic differences. Bioinformatics was used to determine the diversity of genes known or predicted to be involved in biosynthesis of the O-antigenic moiety of LPS in B. pseudomallei and its near-relative species. Multiplex-PCR assays were developed to target diversity of the O-antigen biosynthesis gene patterns or LPS genotypes in B. pseudomallei populations. We found that the typical LPS genotype (LPS genotype A) was highly prevalent in strains from Thailand and other countries in Southeast Asia, whereas the atypical LPS genotype (LPS genotype B) was most often detected in Australian strains (~13.8%). In addition, we report a novel LPS ladder pattern, a derivative of the atypical LPS phenotype, associated with an uncommon O-antigen biosynthesis gene cluster that is found in only a small B. pseudomallei sub-population. This new LPS group was designated as genotype B2. We also report natural mutations in the O-antigen biosynthesis genes that potentially cause the rough LPS phenotype. We postulate that the diversity of LPS may correlate with differential immunopathogenicity and virulence among B. pseudomallei strains.

Original languageEnglish (US)
Article numbere1453
JournalPLoS Neglected Tropical Diseases
Volume6
Issue number1
DOIs
StatePublished - Jan 2012

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Burkholderia pseudomallei
Antigenic Variation
Lipopolysaccharides
Molecular Biology
Genotype
O Antigens
Virulence
Melioidosis
Genes
Phenotype
Southeastern Asia
Multiplex Polymerase Chain Reaction
Thailand
Multigene Family
Computational Biology
Population
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

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The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharide. / Tuanyok, Apichai; Stone, Joshua K.; Mayo, Mark; Kaestli, Mirjam; Gruendike, Jeffrey; Georgia, Shalamar; Warrington, Stephanie; Mullins, Travis; Allender, Christopher J.; Wagner, David M; Chantratita, Narisara; Peacock, Sharon J.; Currie, Bart J.; Keim, Paul S.

In: PLoS Neglected Tropical Diseases, Vol. 6, No. 1, e1453, 01.2012.

Research output: Contribution to journalArticle

Tuanyok, A, Stone, JK, Mayo, M, Kaestli, M, Gruendike, J, Georgia, S, Warrington, S, Mullins, T, Allender, CJ, Wagner, DM, Chantratita, N, Peacock, SJ, Currie, BJ & Keim, PS 2012, 'The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharide', PLoS Neglected Tropical Diseases, vol. 6, no. 1, e1453. https://doi.org/10.1371/journal.pntd.0001453
Tuanyok, Apichai ; Stone, Joshua K. ; Mayo, Mark ; Kaestli, Mirjam ; Gruendike, Jeffrey ; Georgia, Shalamar ; Warrington, Stephanie ; Mullins, Travis ; Allender, Christopher J. ; Wagner, David M ; Chantratita, Narisara ; Peacock, Sharon J. ; Currie, Bart J. ; Keim, Paul S. / The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharide. In: PLoS Neglected Tropical Diseases. 2012 ; Vol. 6, No. 1.
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