The effects of circumcision on the penis microbiome

Lance B. Price, Cindy M. Liu, Kristine E. Johnson, Maliha Aziz, Matthew K. Lau, Jolene Bowers, Jacques Ravel, Paul S Keim, David Serwadda, Maria J. Wawer, Ronald H. Gray

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Background: Circumcision is associated with significant reductions in HIV, HSV-2 and HPV infections among men and significant reductions in bacterial vaginosis among their female partners. Methodology/Principal Findings: We assessed the penile (coronal sulci) microbiota in 12 HIV-negative Ugandan men before and after circumcision. Microbiota were characterized using sequence-tagged 16S rRNA gene pyrosequencing targeting the V3 - V4 hypervariable regions. Taxonomic classification was performed using the RDP Naïve Bayesian Classifier. Among the 42 unique bacterial families identified, Pseudomonadaceae and Oxalobactericeae were the most abundant irrespective of circumcision status. Circumcision was associated with a significant change in the overall microbiota (PerMANOVA p = 0.007) and with a significant decrease in putative anaerobic bacterial families (Wilcoxon Signed-Rank test p = 0.014). Specifically, two families - Clostridiales Family XI (p = 0.006) and Prevotellaceae (p = 0.006) - were uniquely abundant before circumcision. Within these families we identified a number of anaerobic genera previously associated with bacterial vaginosis including: Anaerococcus spp., Finegoldia spp., Peptoniphilus spp., and Prevotella spp. Conclusions/Significance: The anoxic microenvironment of the subpreputial space may support pro-inflammatory anaerobes that can activate Langerhans cells to present HIV to CD4 cells in draining lymph nodes. Thus, the reduction in putative anaerobic bacteria after circumcision may play a role in protection from HIV and other sexually transmitted diseases.

Original languageEnglish (US)
Article numbere8422
JournalPLoS One
Volume5
Issue number1
DOIs
StatePublished - Jan 6 2010

Fingerprint

penis
Microbiota
Penis
Finegoldia
Bacterial Vaginosis
Anaerococcus
Peptoniphilus
HIV
Pseudomonadaceae
Clostridiales
Prevotella
Langerhans cells
sexually transmitted diseases
anaerobes
gene targeting
HIV-2
Human Herpesvirus 2
lymph nodes
Langerhans Cells
Gene Targeting

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Price, L. B., Liu, C. M., Johnson, K. E., Aziz, M., Lau, M. K., Bowers, J., ... Gray, R. H. (2010). The effects of circumcision on the penis microbiome. PLoS One, 5(1), [e8422]. https://doi.org/10.1371/journal.pone.0008422

The effects of circumcision on the penis microbiome. / Price, Lance B.; Liu, Cindy M.; Johnson, Kristine E.; Aziz, Maliha; Lau, Matthew K.; Bowers, Jolene; Ravel, Jacques; Keim, Paul S; Serwadda, David; Wawer, Maria J.; Gray, Ronald H.

In: PLoS One, Vol. 5, No. 1, e8422, 06.01.2010.

Research output: Contribution to journalArticle

Price, LB, Liu, CM, Johnson, KE, Aziz, M, Lau, MK, Bowers, J, Ravel, J, Keim, PS, Serwadda, D, Wawer, MJ & Gray, RH 2010, 'The effects of circumcision on the penis microbiome', PLoS One, vol. 5, no. 1, e8422. https://doi.org/10.1371/journal.pone.0008422
Price LB, Liu CM, Johnson KE, Aziz M, Lau MK, Bowers J et al. The effects of circumcision on the penis microbiome. PLoS One. 2010 Jan 6;5(1). e8422. https://doi.org/10.1371/journal.pone.0008422
Price, Lance B. ; Liu, Cindy M. ; Johnson, Kristine E. ; Aziz, Maliha ; Lau, Matthew K. ; Bowers, Jolene ; Ravel, Jacques ; Keim, Paul S ; Serwadda, David ; Wawer, Maria J. ; Gray, Ronald H. / The effects of circumcision on the penis microbiome. In: PLoS One. 2010 ; Vol. 5, No. 1.
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