The chlamydomonas zygospore

Mutant strains of Chlamydomonas monoica blocked in zygospore morphogenesis comprise 46 complementation groups

Karen P VanWinkle-Swift, Kristin Baron, Alexander McNamara, Peter Minke, Cynthia Burrascano, Janine Maddock

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chlamydomonas monoica undergoes homothallic sexual reproduction in response to nitrogen starvation. Mating pairs are established in clonal culture via flagellar agglutination and fuse by way of activated mating structures to form the quadriflagellate zygote. The zygote further mattures into a dormant diploid zygospore through a series of events that we collectively refer to as zygosporulation. Mutants that arrest development prior to the completion of zygosporulation have been obtained through the use of a variety of mutagens, including ultraviolet irradiation, 5- fluorodeoxyuridine, ethyl methanesulfonate, and methyl methanesulfonate. Complementation analysis indicates that the present mutant collection includes alleles affecting 46 distinct zygote-specific functions. The frequency with which alleles at previously defined loci have been recovered in the most recent mutant searches suggests that as many as 30 additional zygote-specific loci may still remain to be identified. Nevertheless, the present collection should provide a powerful base for ultrastructural, biochemical, and molecular analysis of zygospore morphogenesis and dormancy in Chlamydomonas.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalGenetics
Volume148
Issue number1
StatePublished - Jan 1998

Fingerprint

Chlamydomonas
Zygote
Morphogenesis
Alleles
Floxuridine
Ethyl Methanesulfonate
Methyl Methanesulfonate
Agglutination
Mutagens
Starvation
Diploidy
Reproduction
Nitrogen

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

The chlamydomonas zygospore : Mutant strains of Chlamydomonas monoica blocked in zygospore morphogenesis comprise 46 complementation groups. / VanWinkle-Swift, Karen P; Baron, Kristin; McNamara, Alexander; Minke, Peter; Burrascano, Cynthia; Maddock, Janine.

In: Genetics, Vol. 148, No. 1, 01.1998, p. 131-137.

Research output: Contribution to journalArticle

VanWinkle-Swift, Karen P ; Baron, Kristin ; McNamara, Alexander ; Minke, Peter ; Burrascano, Cynthia ; Maddock, Janine. / The chlamydomonas zygospore : Mutant strains of Chlamydomonas monoica blocked in zygospore morphogenesis comprise 46 complementation groups. In: Genetics. 1998 ; Vol. 148, No. 1. pp. 131-137.
@article{9e311e0140a343b1bdff973408e46ebf,
title = "The chlamydomonas zygospore: Mutant strains of Chlamydomonas monoica blocked in zygospore morphogenesis comprise 46 complementation groups",
abstract = "Chlamydomonas monoica undergoes homothallic sexual reproduction in response to nitrogen starvation. Mating pairs are established in clonal culture via flagellar agglutination and fuse by way of activated mating structures to form the quadriflagellate zygote. The zygote further mattures into a dormant diploid zygospore through a series of events that we collectively refer to as zygosporulation. Mutants that arrest development prior to the completion of zygosporulation have been obtained through the use of a variety of mutagens, including ultraviolet irradiation, 5- fluorodeoxyuridine, ethyl methanesulfonate, and methyl methanesulfonate. Complementation analysis indicates that the present mutant collection includes alleles affecting 46 distinct zygote-specific functions. The frequency with which alleles at previously defined loci have been recovered in the most recent mutant searches suggests that as many as 30 additional zygote-specific loci may still remain to be identified. Nevertheless, the present collection should provide a powerful base for ultrastructural, biochemical, and molecular analysis of zygospore morphogenesis and dormancy in Chlamydomonas.",
author = "VanWinkle-Swift, {Karen P} and Kristin Baron and Alexander McNamara and Peter Minke and Cynthia Burrascano and Janine Maddock",
year = "1998",
month = "1",
language = "English (US)",
volume = "148",
pages = "131--137",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "1",

}

TY - JOUR

T1 - The chlamydomonas zygospore

T2 - Mutant strains of Chlamydomonas monoica blocked in zygospore morphogenesis comprise 46 complementation groups

AU - VanWinkle-Swift, Karen P

AU - Baron, Kristin

AU - McNamara, Alexander

AU - Minke, Peter

AU - Burrascano, Cynthia

AU - Maddock, Janine

PY - 1998/1

Y1 - 1998/1

N2 - Chlamydomonas monoica undergoes homothallic sexual reproduction in response to nitrogen starvation. Mating pairs are established in clonal culture via flagellar agglutination and fuse by way of activated mating structures to form the quadriflagellate zygote. The zygote further mattures into a dormant diploid zygospore through a series of events that we collectively refer to as zygosporulation. Mutants that arrest development prior to the completion of zygosporulation have been obtained through the use of a variety of mutagens, including ultraviolet irradiation, 5- fluorodeoxyuridine, ethyl methanesulfonate, and methyl methanesulfonate. Complementation analysis indicates that the present mutant collection includes alleles affecting 46 distinct zygote-specific functions. The frequency with which alleles at previously defined loci have been recovered in the most recent mutant searches suggests that as many as 30 additional zygote-specific loci may still remain to be identified. Nevertheless, the present collection should provide a powerful base for ultrastructural, biochemical, and molecular analysis of zygospore morphogenesis and dormancy in Chlamydomonas.

AB - Chlamydomonas monoica undergoes homothallic sexual reproduction in response to nitrogen starvation. Mating pairs are established in clonal culture via flagellar agglutination and fuse by way of activated mating structures to form the quadriflagellate zygote. The zygote further mattures into a dormant diploid zygospore through a series of events that we collectively refer to as zygosporulation. Mutants that arrest development prior to the completion of zygosporulation have been obtained through the use of a variety of mutagens, including ultraviolet irradiation, 5- fluorodeoxyuridine, ethyl methanesulfonate, and methyl methanesulfonate. Complementation analysis indicates that the present mutant collection includes alleles affecting 46 distinct zygote-specific functions. The frequency with which alleles at previously defined loci have been recovered in the most recent mutant searches suggests that as many as 30 additional zygote-specific loci may still remain to be identified. Nevertheless, the present collection should provide a powerful base for ultrastructural, biochemical, and molecular analysis of zygospore morphogenesis and dormancy in Chlamydomonas.

UR - http://www.scopus.com/inward/record.url?scp=0031882772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031882772&partnerID=8YFLogxK

M3 - Article

VL - 148

SP - 131

EP - 137

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 1

ER -