Taxonomic patterns in the nitrogen assimilation of soil prokaryotes

Ember M. Morrissey, Rebecca L. Mau, Egbert Schwartz, Benjamin J. Koch, Michaela Hayer, Bruce A Hungate

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Nitrogen (N) is frequently a limiting nutrient in soil; its availability can govern ecosystem functions such as primary production and decomposition. Assimilation of N by microorganisms impacts the availability of N in soil. Despite its established ecological significance, the contributions of microbial taxa to N assimilation are unknown. Here we measure N uptake and use by microbial phylotypes and taxonomic groups within a diverse assemblage of soil microbes through quantitative stable isotope probing (qSIP) with 15N. Following incubation with 15 NH4+, distinct patterns of 15N assimilation among taxonomic groups were observed. For instance, glucose addition stimulated 15N assimilation in most members of Actinobacteria and Proteobacteria but generally decreased 15N use by Firmicutes and Bacteriodetes. While NH4+ is considered a preferred and universal source of N to prokaryotes, the majority (> 80%) of N assimilation in our soils could be attributed to a handful of active orders. Characterizing N assimilation of taxonomic groups with 15N qSIP may provide a basis for understanding how microbial community composition influences N availability in the environment.

Original languageEnglish (US)
JournalEnvironmental Microbiology
DOIs
StateAccepted/In press - Jan 1 2018

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prokaryote
prokaryotic cells
stable isotopes
Nitrogen
Soil
Firmicutes
Actinobacteria
nitrogen
Proteobacteria
soil microorganisms
Isotopes
soil nutrients
microbial communities
primary productivity
soil
microorganisms
glucose
degradation
ecosystems
stable isotope

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Taxonomic patterns in the nitrogen assimilation of soil prokaryotes. / Morrissey, Ember M.; Mau, Rebecca L.; Schwartz, Egbert; Koch, Benjamin J.; Hayer, Michaela; Hungate, Bruce A.

In: Environmental Microbiology, 01.01.2018.

Research output: Contribution to journalArticle

Morrissey, Ember M. ; Mau, Rebecca L. ; Schwartz, Egbert ; Koch, Benjamin J. ; Hayer, Michaela ; Hungate, Bruce A. / Taxonomic patterns in the nitrogen assimilation of soil prokaryotes. In: Environmental Microbiology. 2018.
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