Stage-specific antigens of Nematospiroides dubius Baylis, 1926 (Nematoda

Heligmosomides).

J. H. Adams, I. J. East, Fernando P Monroy, E. A. Washington, C. Dobson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Antigens were identified from Nematospiroides dubius recovered from outbred Quackenbush mice between 4 and 10 days postinoculation (PI). Parasite surface proteins were radioiodinated and extracts of the whole worms were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and reacted with normal and immune mouse sera followed by an avidin-biotin-peroxidase assay. Antigens ranged between 250,000 and 20,000 molecular weight (MW). A major surface antigen, 60,000 MW, which appeared to be a complex of different antigens, and a 250,000-MW internal antigen were found on fourth-stage (L4) and fifth-stage (L5) larvae 5-10 days PI but not earlier. A group of minor surface antigens (24,000-30,000 MW) were also expressed as larvae molted from L4 to L5, 6 and 7 days PI, but they differed from antigens of similar MW expressed by adult worms. An antigen, 45,000 MW, was detected in worms 5-10 days PI, but it was only expressed on the surface of L5 worms 9 and 10 days PI. We suggest that the antigen(s) common to adults and larvae may account for protective immunity.

Original languageEnglish (US)
Pages (from-to)1164-1168
Number of pages5
JournalJournal of Parasitology
Volume73
Issue number6
StatePublished - Dec 1987
Externally publishedYes

Fingerprint

Nematospiroides dubius
Heligmosomoides polygyrus
Nematoda
antigen
antigens
Molecular Weight
Antigens
molecular weight
Larva
surface antigens
Surface Antigens
larvae
larva
avidin
Collodion
Avidin
mice
surface proteins
biotin
Biotin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology
  • Parasitology

Cite this

Adams, J. H., East, I. J., Monroy, F. P., Washington, E. A., & Dobson, C. (1987). Stage-specific antigens of Nematospiroides dubius Baylis, 1926 (Nematoda: Heligmosomides). Journal of Parasitology, 73(6), 1164-1168.

Stage-specific antigens of Nematospiroides dubius Baylis, 1926 (Nematoda : Heligmosomides). / Adams, J. H.; East, I. J.; Monroy, Fernando P; Washington, E. A.; Dobson, C.

In: Journal of Parasitology, Vol. 73, No. 6, 12.1987, p. 1164-1168.

Research output: Contribution to journalArticle

Adams, JH, East, IJ, Monroy, FP, Washington, EA & Dobson, C 1987, 'Stage-specific antigens of Nematospiroides dubius Baylis, 1926 (Nematoda: Heligmosomides).', Journal of Parasitology, vol. 73, no. 6, pp. 1164-1168.
Adams, J. H. ; East, I. J. ; Monroy, Fernando P ; Washington, E. A. ; Dobson, C. / Stage-specific antigens of Nematospiroides dubius Baylis, 1926 (Nematoda : Heligmosomides). In: Journal of Parasitology. 1987 ; Vol. 73, No. 6. pp. 1164-1168.
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