Stable isotope probing with 18O-water to investigate growth and mortality of ammonia oxidizing bacteria and archaea in soil

Karen Adair, Egbert Schwartz

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Ammonia oxidizing bacteria (AOB) and archaea oxidize ammonia to nitrite, the first and rate-limiting step in the important ecosystem process of nitrification. Growth and mortality of ammonia oxidizers in soil are difficult to quantify but accurate measurements would offer important insights into how environmental parameters regulate the population dynamics of these organisms. Stable isotope probing (SIP) is a recently developed technique that can identify microorganisms that assimilate labeled substrates and can be adapted to quantify the growth of organisms in soil. Here, we describe the use of SIP with 18O-water to investigate the growth and mortality of ammonia oxidizers in a soil taken from a ponderosa pine forest in northern Arizona, USA. Addition of ammonia to soil stimulated the growth of AOB but not ammonia oxidizing archaea (AOA). The mortality of AOA was increased upon addition of ammonia to soil; however, the variance in these measurements was high. The mortality of AOB, in contrast, was not impacted by addition of ammonia to soil. The results suggest that increased ammonia availability in soil favors AOB over AOA.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages155-169
Number of pages15
EditionC
DOIs
StatePublished - Jan 1 2011

Publication series

NameMethods in Enzymology
NumberC
Volume486
ISSN (Print)0076-6879

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Keywords

  • Ammonia oxidizing archaea
  • Ammonia oxidizing bacteria
  • Nitrification
  • Stable isotope probing

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Adair, K., & Schwartz, E. (2011). Stable isotope probing with 18O-water to investigate growth and mortality of ammonia oxidizing bacteria and archaea in soil. In Methods in Enzymology (C ed., pp. 155-169). (Methods in Enzymology; Vol. 486, No. C). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-381294-0.00007-9