Stable Isotope Probing of Microorganisms in Environmental Samples with H218O

Egbert Schwartz, Michaela Hayer, Bruce A Hungate, Rebecca L. Mau

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We describe a protocol for investigating microbial growth in environmental samples via stable isotope probing (SIP) with H218O. Water is a universal substrate for all microorganisms and replication is required for DNA to become labeled with 18O. By measuring how much the DNA of each taxon becomes enriched with 18O when an environmental sample is incubated with H218O, it is feasible to quantify that population’s DNA replication rate, which is a proxy for growth.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages129-136
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2046
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

    Fingerprint

Keywords

  • HO
  • Microbial growth
  • Stable isotope probing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Schwartz, E., Hayer, M., Hungate, B. A., & Mau, R. L. (2019). Stable Isotope Probing of Microorganisms in Environmental Samples with H218O. In Methods in Molecular Biology (pp. 129-136). (Methods in Molecular Biology; Vol. 2046). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9721-3_10