Stable isotope discrimination during soil denitrification: Production and consumption of nitrous oxide

Oleg V. Menyailo, Bruce A Hungate

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Measuring the stable isotope composition of nitrous oxide (N2O) evolved from soil could improve our understanding of the relative contributions of the main microbial processes (nitrification and denitrification) responsible for N2O formation in soil. However, interpretation of the isotopic data in N2O is complicated by the lack of knowledge of fractionation parameters by different microbial processes responsible for N2O production and consumption. Here we report isotopic enrichment for both nitrogen and oxygen isotopes in two stages of denitrification, N2O production and N2O reduction. We found that during both N2O production and reduction, enrichments were higher for oxygen than nitrogen. For both elements, enrichments were larger for N2O production stage than for N2O reduction. During gross N2O production, the ratio of δ18 O-to-δ15N differed between soils, ranging from 1.6 to 2.7. By contrast, during N2O reduction, we observed a constant ratio of δ18 O-to-δ15N with a value near 2.5. If general, this ratio could be used to estimate the proportion of N2O being reduced in the soil before escaping into the atmosphere. Because N2O-reductase enriches N2O in both isotopes, the global reduction of N2O consumption by soil may contribute to the globally observed isotopic depletion of atmospheric N 2O.

Original languageEnglish (US)
Article numberGB3025
JournalGlobal Biogeochemical Cycles
Volume20
Issue number3
DOIs
StatePublished - Sep 2006

Fingerprint

Denitrification
Nitrous Oxide
nitrous oxide
Isotopes
denitrification
stable isotope
Soils
soil
Nitrogen Isotopes
Oxygen Isotopes
Nitrification
nitrogen isotope
Fractionation
oxygen isotope
nitrification
Oxidoreductases
Nitrogen
fractionation
consumption
isotope

ASJC Scopus subject areas

  • Global and Planetary Change
  • Atmospheric Science
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Stable isotope discrimination during soil denitrification : Production and consumption of nitrous oxide. / Menyailo, Oleg V.; Hungate, Bruce A.

In: Global Biogeochemical Cycles, Vol. 20, No. 3, GB3025, 09.2006.

Research output: Contribution to journalArticle

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