Spotted bat (Euderma maculatum) microsatellite discovery using illumina sequencing

Faith M. Walker, Jeffrey T Foster, Kevin P. Drees, Carol L Chambers

Research output: Contribution to journalArticle

Abstract

The spotted bat (Euderma maculatum) is a rarely-encountered species for which behavior and population attributes are largely unknown. Using next-generation sequencing, we identified and characterized 17 microsatellite loci, which were screened for 31 individuals from northern Arizona. Allelic diversity, observed heterozygosity, and power of discrimination were high (NA: 5-8 alleles per locus; HO: 0.55-0.90; PID: 1.2 × 10-15). All loci were in HWE, there was no evidence of null alleles or linkage disequilibrium, and five loci amplified and were variable in another Vespertillionid (Eptesicus fuscus). We will use these loci to evaluate gene flow and genetic diversity across the range of the spotted bat and determine population size in northern Arizona. The latter information is important to resource managers, who attempt to set mortality thresholds for bats at wind energy facilities in this region.

Original languageEnglish (US)
Pages (from-to)457-459
Number of pages3
JournalConservation Genetics Resources
Volume6
Issue number2
DOIs
StatePublished - 2014

Fingerprint

bat
Microsatellite Repeats
Chiroptera
microsatellite repeats
loci
allele
Alleles
Gene Flow
Linkage Disequilibrium
Population Density
disequilibrium
heterozygosity
gene flow
population size
wind power
null alleles
mortality
linkage disequilibrium
Mortality
resource

Keywords

  • Euderma maculatum
  • Microsatellite
  • Next-generation sequencing
  • Spotted bat

ASJC Scopus subject areas

  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Spotted bat (Euderma maculatum) microsatellite discovery using illumina sequencing. / Walker, Faith M.; Foster, Jeffrey T; Drees, Kevin P.; Chambers, Carol L.

In: Conservation Genetics Resources, Vol. 6, No. 2, 2014, p. 457-459.

Research output: Contribution to journalArticle

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