Skeletal muscle fiber cross-sectional area

Effects of freezing procedures

R. R. Roy, David J Pierotti, V. R. Edgerton

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The cross-sectional area (CSA) of individual fibers is an important measure of skeletal muscle plasticity to investigate the effects of different freezing procedures on CSA measurements, the CSA of type-identified fibers in the cat tibialis anterior were determined following quick-freezing the muscle at a fixed physiological length (ipsilateral, frozen at length) and compared to the fiber CSA following quick-freezing a mid-portion of the muscle where the fibers were allowed to freely shorten during tissue preparation (contralateral, frozen as a block). The mean CSA of each fiber type was significantly smaller in the muscles frozen at length vs. frozen as a block in both a deep' (close to the bone) and a superficial (away from the bone) region of the muscle, except for the slow oxidative dative (SO) fibers in the superficial region. The percent difference in mean fiber CSA was smaller for the SO compared to the fast oxidative glycolytic and fast glycolytic fibers in both the deep (41, 47 and 56%, respectively) and superficial (20, 36 and 48%, respectively) regions. In addition, the differences in the mean CSAs of the fast fibers between the two freezing procedures were ~10% larger in the deep compared to the superficial region. The fiber-type differential responses may be related, at least in part, to the architectural features of the fibers. These data indicate that the freezing procedures used to prepare the muscle tissue are an important consideration when determining the CSA of individual skeletal muscle fibers and consequently the specific tension, tension per unit CSA, of the muscle units.

Original languageEnglish (US)
Pages (from-to)131-135
Number of pages5
JournalActa Anatomica
Volume155
Issue number2
StatePublished - 1996
Externally publishedYes

Fingerprint

Skeletal Muscle Fibers
Freezing
Muscles
Bone and Bones
Skeletal Muscle
Cats

Keywords

  • Cat
  • Fiber cross-sectional area
  • Fiber types
  • Freezing procedures
  • Muscle fiber architecture
  • Skeletal muscle
  • Specific tension

ASJC Scopus subject areas

  • Anatomy

Cite this

Skeletal muscle fiber cross-sectional area : Effects of freezing procedures. / Roy, R. R.; Pierotti, David J; Edgerton, V. R.

In: Acta Anatomica, Vol. 155, No. 2, 1996, p. 131-135.

Research output: Contribution to journalArticle

Roy, R. R. ; Pierotti, David J ; Edgerton, V. R. / Skeletal muscle fiber cross-sectional area : Effects of freezing procedures. In: Acta Anatomica. 1996 ; Vol. 155, No. 2. pp. 131-135.
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