TY - JOUR
T1 - Sequencing our way towards understanding global eukaryotic biodiversity
AU - Bik, Holly M.
AU - Porazinska, Dorota L.
AU - Creer, Simon
AU - Caporaso, J. Gregory
AU - Knight, Rob
AU - Thomas, W. Kelley
N1 - Funding Information:
The authors would like to thank the anonymous reviewers for their insightful comments that significantly helped to improve an earlier version of the manuscript. Development of this manuscript was made possible by a Catalysis Meeting award (HB and WKT) from the National Evolutionary Synthesis Center. HB and WKT supported through NSF (DEB-1058458 and NIH (NIH-1P20RR030360-01)). SC supported by a Natural Environment Research Council (NERC) New Investigator Grant (NE/E001505/1), a Post Genomic and Proteomics Grant (NE/F001266/1) and a Molecular Genetics Facility Grant (MGF-167). DP acknowledges funding from USDA/CSREES – TSTAR (grants 2006-04347 and 2008-34135-19505), NSF (DEB-0450537) and the CR-USA Foundation. RK and JGC supported in part by the National Institutes of Health, Bill and Melinda Gates Foundation, the Crohns and Colitis Foundation of America, the Sloan Indoor Environment program and the Howard Hughes Medical Institute.
PY - 2012/4
Y1 - 2012/4
N2 - Microscopic eukaryotes are abundant, diverse and fill critical ecological roles across every ecosystem on Earth, yet there is a well-recognized gap in understanding of their global biodiversity. Fundamental advances in DNA sequencing and bioinformatics now allow accurate en masse biodiversity assessments of microscopic eukaryotes from environmental samples. Despite a promising outlook, the field of eukaryotic marker gene surveys faces significant challenges: how to generate data that are most useful to the community, especially in the face of evolving sequencing technologies and bioinformatics pipelines, and how to incorporate an expanding number of target genes.
AB - Microscopic eukaryotes are abundant, diverse and fill critical ecological roles across every ecosystem on Earth, yet there is a well-recognized gap in understanding of their global biodiversity. Fundamental advances in DNA sequencing and bioinformatics now allow accurate en masse biodiversity assessments of microscopic eukaryotes from environmental samples. Despite a promising outlook, the field of eukaryotic marker gene surveys faces significant challenges: how to generate data that are most useful to the community, especially in the face of evolving sequencing technologies and bioinformatics pipelines, and how to incorporate an expanding number of target genes.
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U2 - 10.1016/j.tree.2011.11.010
DO - 10.1016/j.tree.2011.11.010
M3 - Review article
C2 - 22244672
AN - SCOPUS:84858617141
VL - 27
SP - 233
EP - 243
JO - Trends in Ecology and Evolution
JF - Trends in Ecology and Evolution
SN - 0169-5347
IS - 4
ER -