Scaffold-based three-dimensional human fibroblast culture provides a structural matrix that supports angiogenesis in infarcted heart tissue

Robert S Kellar, Lee K. Landeen, Benjamin R. Shepherd, Gail K. Naughton, Anthony Ratcliffe, Stuart K. Williams

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Background - We have developed techniques to implant angiogenic patches onto the epicardium over regions of infarcted cardiac tissue to stimulate revascularization of the damaged tissue. These experiments used a scaffold-based 3D human dermal fibroblast culture (3DFC) as an epicardial patch. The 3DFC contains viable cells that secrete angiogenic growth factors and has previously been shown to stimulate angiogenic activity. The hypothesis tested was that a viable 3DFC cardiac patch would stimulate an angiogenic response within an area of infarcted cardiac tissue. Methods and Results - A coronary occlusion of a branch of the left anterior descending coronary artery was performed by thermal ligation in severe combined immunodeficient mice. 3DFCs with or without viable cells were sized to the damaged area, implanted in replicate mice onto the epicardium at the site of tissue injury, and compared with animals that received infarct surgery but no implant. Fourteen and 30 days after surgery, hearts were exposed and photographed, and tissue samples were prepared for histology and cytochemistry. Fourteen and 30 days after surgery, the damaged myocardium receiving viable 3DFC exhibited a significantly greater angiogenic response (including arterioles, venules, and capillaries) than nonviable and untreated control groups. Conclusions - In this animal model, viable 3DFC stimulates angiogenesis within a region of cardiac infarction and can augment a repair response in damaged tissue. Therefore, a potential use for 3DFC is the repair of myocardial tissue damaged by infarction.

Original languageEnglish (US)
Pages (from-to)2063-2068
Number of pages6
JournalCirculation
Volume104
Issue number17
StatePublished - Oct 23 2001
Externally publishedYes

Fingerprint

Fibroblasts
Skin
Pericardium
Ambulatory Surgical Procedures
Infarction
Histocytochemistry
SCID Mice
Venules
Angiogenesis Inducing Agents
Coronary Occlusion
Arterioles
Ligation
Intercellular Signaling Peptides and Proteins
Coronary Vessels
Myocardium
Histology
Animal Models
Hot Temperature
Control Groups
Wounds and Injuries

Keywords

  • Angiogenesis
  • Ischemia
  • Myocardial infarction
  • Revascularization

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Kellar, R. S., Landeen, L. K., Shepherd, B. R., Naughton, G. K., Ratcliffe, A., & Williams, S. K. (2001). Scaffold-based three-dimensional human fibroblast culture provides a structural matrix that supports angiogenesis in infarcted heart tissue. Circulation, 104(17), 2063-2068.

Scaffold-based three-dimensional human fibroblast culture provides a structural matrix that supports angiogenesis in infarcted heart tissue. / Kellar, Robert S; Landeen, Lee K.; Shepherd, Benjamin R.; Naughton, Gail K.; Ratcliffe, Anthony; Williams, Stuart K.

In: Circulation, Vol. 104, No. 17, 23.10.2001, p. 2063-2068.

Research output: Contribution to journalArticle

Kellar, RS, Landeen, LK, Shepherd, BR, Naughton, GK, Ratcliffe, A & Williams, SK 2001, 'Scaffold-based three-dimensional human fibroblast culture provides a structural matrix that supports angiogenesis in infarcted heart tissue', Circulation, vol. 104, no. 17, pp. 2063-2068.
Kellar, Robert S ; Landeen, Lee K. ; Shepherd, Benjamin R. ; Naughton, Gail K. ; Ratcliffe, Anthony ; Williams, Stuart K. / Scaffold-based three-dimensional human fibroblast culture provides a structural matrix that supports angiogenesis in infarcted heart tissue. In: Circulation. 2001 ; Vol. 104, No. 17. pp. 2063-2068.
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