Role of glucocorticoids and Toxoplasma gondii infection on murine intestinal epithelial cells

Stacy L. Johnson, Radha Gopal, Amber Enriquez, Fernando P Monroy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Glucocorticoids (GCs) are stress hormones secreted in response to perceived psychological and or physiological stress. GCs have been shown to reduce tissue inflammation by down-regulating the production of inflammatory chemokines produced by epithelial cells. The protozoan parasite Toxoplasma gondii is known to increase cytokine, chemokine, and Toll-like receptors (TLRs) expression in parasite infected mouse intestinal epithelial cells (IECs). We sought to analyze the role of an anti-inflammatory protein, glucocorticoid-induced leucine zipper (GILZ) in MODE-K cells during infection with T. gondii. GILZ expression in MODE-K cells was assessed by PCR and immunoblotting after stimulation with GCs (corticosterone, CORT) or T. gondii infection. GILZ mRNA was constitutively expressed in MODE-K cells but not its protein product. While infection and pre-exposure to CORT decreased GILZ isoforms of 28 and 17. kD, the presence of CORT during infection increased levels of 17. kD isoform. Infected cells treated with CORT had decreased expression of chemokines (IP-10/CXCL10, MCP-1/CCL2, MIP-2/CXCL8) while their expression was increased when endogenous GILZ was removed by siRNA treatment. GILZ up-regulation during infection may serve as a mechanism to decrease epithelial cell responses and facilitate parasite replication.

Original languageEnglish (US)
Pages (from-to)687-694
Number of pages8
JournalParasitology International
Volume63
Issue number5
DOIs
StatePublished - 2014

Fingerprint

Toxoplasmosis
Glucocorticoids
Leucine Zippers
Epithelial Cells
Corticosterone
Chemokines
Parasites
Protein Isoforms
Infection
Physiological Stress
Toll-Like Receptors
Toxoplasma
Psychological Stress
Immunoblotting
Small Interfering RNA
Proteins
Anti-Inflammatory Agents
Up-Regulation
Hormones
Cytokines

Keywords

  • GILZ
  • Glucocorticoids
  • Immunity
  • MODE-K
  • SiRNA
  • Toxoplasma

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Medicine(all)

Cite this

Role of glucocorticoids and Toxoplasma gondii infection on murine intestinal epithelial cells. / Johnson, Stacy L.; Gopal, Radha; Enriquez, Amber; Monroy, Fernando P.

In: Parasitology International, Vol. 63, No. 5, 2014, p. 687-694.

Research output: Contribution to journalArticle

Johnson, Stacy L. ; Gopal, Radha ; Enriquez, Amber ; Monroy, Fernando P. / Role of glucocorticoids and Toxoplasma gondii infection on murine intestinal epithelial cells. In: Parasitology International. 2014 ; Vol. 63, No. 5. pp. 687-694.
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