RFLP mapping using near-isogenic lines in the soybean [Glycine max (L.) Merr.]

G. J. Muehlbauer, P. E. Staswick, J. E. Specht, G. L. Graef, R. C. Shoemaker, Paul S Keim

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A molecular marker analysis of a near-isogenic line (NIL), its donor parent (DP), and its recurrent parent (RP) can provide information about linkages between molecular markers and a conventional marker introgressed into the NIL. If the DP and RP possess different alleles for a given molecular marker, and if the NIL possesses the same allele as the DP, then it is reasonable to presume a linkage between that molecular marker and the introgressed marker. In this study, we examined the utility of RFLPs as molecular markers for the NIL genemapping approach. The allelic status of fifteen RFLP loci was determined in 116 soybean RP/NIL/DP line sets; 66 of the 'Clark' RP type and 50 of the 'Harosoy' RP type. Of the 1740 possible allelic comparisons (116 NILs x 15 RFLP loci), 1638 were tested and 462 (33.9%) of those were informative (i.e., the RP and DP had different RFLP alleles). In 15 (3.2%) of these 462 cases the NIL possessed the DP-derived RFLP allele, leading to a presumption of linkage between the RFLP locus and the introgressed conventional marker locus. Two presumptive linkages, pK-3 - and pK-472 - L fi, were subsequently confirmed by cosegregation linkage analysis. Although not yet confirmed, two other associations, pk-7 ab and pK-229 - y9 seemed to be plausible linkages, primarily because the pk-7 - ab association was detected in two independently derived NILs and both markers of the pK-229 - y9 association were known to be linked to Pb. The data obtained in this investigation indicated that RFLP loci were useful molecular markers for the NIL gene-mapping technique.

Original languageEnglish (US)
Pages (from-to)189-198
Number of pages10
JournalTheoretical And Applied Genetics
Volume81
Issue number2
DOIs
StatePublished - Feb 1991
Externally publishedYes

Fingerprint

isogenic lines
Soybeans
Restriction Fragment Length Polymorphisms
Glycine max
restriction fragment length polymorphism
linkage (genetics)
soybeans
genetic markers
loci
Alleles
alleles
Chromosome Mapping
chromosome mapping

Keywords

  • Genetic map
  • Glycine max
  • Linkage
  • Molecular markers
  • Near-isogenic lines
  • RFLP

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Muehlbauer, G. J., Staswick, P. E., Specht, J. E., Graef, G. L., Shoemaker, R. C., & Keim, P. S. (1991). RFLP mapping using near-isogenic lines in the soybean [Glycine max (L.) Merr.]. Theoretical And Applied Genetics, 81(2), 189-198. https://doi.org/10.1007/BF00215722

RFLP mapping using near-isogenic lines in the soybean [Glycine max (L.) Merr.]. / Muehlbauer, G. J.; Staswick, P. E.; Specht, J. E.; Graef, G. L.; Shoemaker, R. C.; Keim, Paul S.

In: Theoretical And Applied Genetics, Vol. 81, No. 2, 02.1991, p. 189-198.

Research output: Contribution to journalArticle

Muehlbauer, GJ, Staswick, PE, Specht, JE, Graef, GL, Shoemaker, RC & Keim, PS 1991, 'RFLP mapping using near-isogenic lines in the soybean [Glycine max (L.) Merr.]', Theoretical And Applied Genetics, vol. 81, no. 2, pp. 189-198. https://doi.org/10.1007/BF00215722
Muehlbauer, G. J. ; Staswick, P. E. ; Specht, J. E. ; Graef, G. L. ; Shoemaker, R. C. ; Keim, Paul S. / RFLP mapping using near-isogenic lines in the soybean [Glycine max (L.) Merr.]. In: Theoretical And Applied Genetics. 1991 ; Vol. 81, No. 2. pp. 189-198.
@article{8be3d70a865a4aa6bf04ccf0067de0bc,
title = "RFLP mapping using near-isogenic lines in the soybean [Glycine max (L.) Merr.]",
abstract = "A molecular marker analysis of a near-isogenic line (NIL), its donor parent (DP), and its recurrent parent (RP) can provide information about linkages between molecular markers and a conventional marker introgressed into the NIL. If the DP and RP possess different alleles for a given molecular marker, and if the NIL possesses the same allele as the DP, then it is reasonable to presume a linkage between that molecular marker and the introgressed marker. In this study, we examined the utility of RFLPs as molecular markers for the NIL genemapping approach. The allelic status of fifteen RFLP loci was determined in 116 soybean RP/NIL/DP line sets; 66 of the 'Clark' RP type and 50 of the 'Harosoy' RP type. Of the 1740 possible allelic comparisons (116 NILs x 15 RFLP loci), 1638 were tested and 462 (33.9{\%}) of those were informative (i.e., the RP and DP had different RFLP alleles). In 15 (3.2{\%}) of these 462 cases the NIL possessed the DP-derived RFLP allele, leading to a presumption of linkage between the RFLP locus and the introgressed conventional marker locus. Two presumptive linkages, pK-3 - and pK-472 - L fi, were subsequently confirmed by cosegregation linkage analysis. Although not yet confirmed, two other associations, pk-7 ab and pK-229 - y9 seemed to be plausible linkages, primarily because the pk-7 - ab association was detected in two independently derived NILs and both markers of the pK-229 - y9 association were known to be linked to Pb. The data obtained in this investigation indicated that RFLP loci were useful molecular markers for the NIL gene-mapping technique.",
keywords = "Genetic map, Glycine max, Linkage, Molecular markers, Near-isogenic lines, RFLP",
author = "Muehlbauer, {G. J.} and Staswick, {P. E.} and Specht, {J. E.} and Graef, {G. L.} and Shoemaker, {R. C.} and Keim, {Paul S}",
year = "1991",
month = "2",
doi = "10.1007/BF00215722",
language = "English (US)",
volume = "81",
pages = "189--198",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - RFLP mapping using near-isogenic lines in the soybean [Glycine max (L.) Merr.]

AU - Muehlbauer, G. J.

AU - Staswick, P. E.

AU - Specht, J. E.

AU - Graef, G. L.

AU - Shoemaker, R. C.

AU - Keim, Paul S

PY - 1991/2

Y1 - 1991/2

N2 - A molecular marker analysis of a near-isogenic line (NIL), its donor parent (DP), and its recurrent parent (RP) can provide information about linkages between molecular markers and a conventional marker introgressed into the NIL. If the DP and RP possess different alleles for a given molecular marker, and if the NIL possesses the same allele as the DP, then it is reasonable to presume a linkage between that molecular marker and the introgressed marker. In this study, we examined the utility of RFLPs as molecular markers for the NIL genemapping approach. The allelic status of fifteen RFLP loci was determined in 116 soybean RP/NIL/DP line sets; 66 of the 'Clark' RP type and 50 of the 'Harosoy' RP type. Of the 1740 possible allelic comparisons (116 NILs x 15 RFLP loci), 1638 were tested and 462 (33.9%) of those were informative (i.e., the RP and DP had different RFLP alleles). In 15 (3.2%) of these 462 cases the NIL possessed the DP-derived RFLP allele, leading to a presumption of linkage between the RFLP locus and the introgressed conventional marker locus. Two presumptive linkages, pK-3 - and pK-472 - L fi, were subsequently confirmed by cosegregation linkage analysis. Although not yet confirmed, two other associations, pk-7 ab and pK-229 - y9 seemed to be plausible linkages, primarily because the pk-7 - ab association was detected in two independently derived NILs and both markers of the pK-229 - y9 association were known to be linked to Pb. The data obtained in this investigation indicated that RFLP loci were useful molecular markers for the NIL gene-mapping technique.

AB - A molecular marker analysis of a near-isogenic line (NIL), its donor parent (DP), and its recurrent parent (RP) can provide information about linkages between molecular markers and a conventional marker introgressed into the NIL. If the DP and RP possess different alleles for a given molecular marker, and if the NIL possesses the same allele as the DP, then it is reasonable to presume a linkage between that molecular marker and the introgressed marker. In this study, we examined the utility of RFLPs as molecular markers for the NIL genemapping approach. The allelic status of fifteen RFLP loci was determined in 116 soybean RP/NIL/DP line sets; 66 of the 'Clark' RP type and 50 of the 'Harosoy' RP type. Of the 1740 possible allelic comparisons (116 NILs x 15 RFLP loci), 1638 were tested and 462 (33.9%) of those were informative (i.e., the RP and DP had different RFLP alleles). In 15 (3.2%) of these 462 cases the NIL possessed the DP-derived RFLP allele, leading to a presumption of linkage between the RFLP locus and the introgressed conventional marker locus. Two presumptive linkages, pK-3 - and pK-472 - L fi, were subsequently confirmed by cosegregation linkage analysis. Although not yet confirmed, two other associations, pk-7 ab and pK-229 - y9 seemed to be plausible linkages, primarily because the pk-7 - ab association was detected in two independently derived NILs and both markers of the pK-229 - y9 association were known to be linked to Pb. The data obtained in this investigation indicated that RFLP loci were useful molecular markers for the NIL gene-mapping technique.

KW - Genetic map

KW - Glycine max

KW - Linkage

KW - Molecular markers

KW - Near-isogenic lines

KW - RFLP

UR - http://www.scopus.com/inward/record.url?scp=0001409435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001409435&partnerID=8YFLogxK

U2 - 10.1007/BF00215722

DO - 10.1007/BF00215722

M3 - Article

C2 - 24221202

AN - SCOPUS:0001409435

VL - 81

SP - 189

EP - 198

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 2

ER -