Restriction fragment length polymorphisms as genetic markers in soybean, Glycine max (L.) merrill

N. R. Apuya, B. L. Frazier, Paul S Keim, E. Jill Roth, K. G. Lark

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Restriction Fragment Length Polymorphisms (RFLP) have been identified between widely distant cultivars ('Minsoy' and 'Noir 1 ') of soybean Glycine max (L.) Merrill. Using as probes randomly chosen clones of DNA, one in five probes revealed a polymorphism. More than half of these polymorphisms appear to result from rearrangements of the genomic DNA. Twenty seven markers were analyzed for linkage in F2 plants. Eleven of these markers were contained in four linkage groups. Five cultivars were compared in a search for new alleles. When RFLP markers corresponding to low copy DNA were used to analyze three other cultivars - 'Sooty', 'Forrest' and 'Mandarin (Ottawa)' - few new alleles were found. Using these probes, five different markers could be used to differentiate the five cultivars. Complex probes, which correspond to repeated DNA, revealed different polymorphisms in different cultivars and a single such probe could be used to distinguish the five cultivars from each other.

Original languageEnglish (US)
Pages (from-to)889-901
Number of pages13
JournalTheoretical And Applied Genetics
Volume75
Issue number6
DOIs
StatePublished - Jun 1988
Externally publishedYes

Fingerprint

Genetic Markers
Soybeans
Restriction Fragment Length Polymorphisms
Glycine max
restriction fragment length polymorphism
soybeans
genetic markers
DNA
cultivars
Alleles
Gene Rearrangement
genetic polymorphism
Clone Cells
alleles
mandarins
linkage groups
linkage (genetics)
clones
genomics

Keywords

  • Allele
  • Genetics
  • Restriction fragment length polymorphism
  • Soybean
  • Variation

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Restriction fragment length polymorphisms as genetic markers in soybean, Glycine max (L.) merrill. / Apuya, N. R.; Frazier, B. L.; Keim, Paul S; Jill Roth, E.; Lark, K. G.

In: Theoretical And Applied Genetics, Vol. 75, No. 6, 06.1988, p. 889-901.

Research output: Contribution to journalArticle

Apuya, N. R. ; Frazier, B. L. ; Keim, Paul S ; Jill Roth, E. ; Lark, K. G. / Restriction fragment length polymorphisms as genetic markers in soybean, Glycine max (L.) merrill. In: Theoretical And Applied Genetics. 1988 ; Vol. 75, No. 6. pp. 889-901.
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