Requirement of yeast fimbrin for actin organization and morphogenesis in vivo

Alison Adams, David Botstein, David G. Drubin

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

The SAC6 gene was found by suppression of a yeast actin mutation1. Its protein product, Sac6p (previously referred to as ABP67), was independently isolated by actin-filament affinity chromatography and colocalizes with actin in vivo2. Thus Sac6p binds to actin in vitro, and functionally associates with actin structures involved in the development and maintenance of cell polarity in vivo2,3. We report here that Sac6p is an actin-filament bundling protein 43% identical in amino-acid sequence to the vertebrate bundling protein fimbrin4. This yeast fimbrin homologue contains two putative actin-binding regions5 homologous to domains of dystrophin, β-spectrin, filamin, actin-gelation protein and α-actinin. Mutants lacking Sac6p do not form normal actin structures and are defective in morphogenesis. These findings demonstrate an in vivo role for the well-documented biochemical interaction between fimbrin and actin.

Original languageEnglish (US)
Pages (from-to)404-408
Number of pages5
JournalNature
Volume354
Issue number6352
StatePublished - Dec 5 1991
Externally publishedYes

Fingerprint

Morphogenesis
Actins
Yeasts
Filamins
Actinin
Spectrin
Cell Polarity
Dystrophin
plastin
Actin Cytoskeleton
Affinity Chromatography
Vertebrates
Amino Acid Sequence
Proteins
Maintenance
Genes

ASJC Scopus subject areas

  • General

Cite this

Adams, A., Botstein, D., & Drubin, D. G. (1991). Requirement of yeast fimbrin for actin organization and morphogenesis in vivo. Nature, 354(6352), 404-408.

Requirement of yeast fimbrin for actin organization and morphogenesis in vivo. / Adams, Alison; Botstein, David; Drubin, David G.

In: Nature, Vol. 354, No. 6352, 05.12.1991, p. 404-408.

Research output: Contribution to journalArticle

Adams, A, Botstein, D & Drubin, DG 1991, 'Requirement of yeast fimbrin for actin organization and morphogenesis in vivo', Nature, vol. 354, no. 6352, pp. 404-408.
Adams, Alison ; Botstein, David ; Drubin, David G. / Requirement of yeast fimbrin for actin organization and morphogenesis in vivo. In: Nature. 1991 ; Vol. 354, No. 6352. pp. 404-408.
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