Quantitative stable isotope probing with H2 18O reveals that most bacterial taxa in soil synthesize new ribosomal RNA

Katerina Papp, Rebecca L. Mau, Michaela Hayer, Benjamin J. Koch, Bruce A. Hungate, Egbert Schwartz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Most soil bacterial taxa are thought to be dormant, or inactive, yet the extent to which they synthetize new rRNA is poorly understood. We analyzed 18O composition of RNA extracted from soil incubated with H2 18O and used quantitative stable isotope probing to characterize rRNA synthesis among microbial taxa. RNA was not fully labeled with 18O, peaking at a mean of 23.6 ± 6.8 atom percent excess (APE) 18O after eight days of incubation, suggesting some ribonucleotides in soil were more than eight days old. Microbial taxa varied in the degree they incorporated 18O into their rRNA over time and there was no correlation between the APE18O of bacterial rRNA and their rRNA to DNA ratios, suggesting that the ratios were not appropriate to measure ribonucleotide synthesis. Our study indicates that, on average, 94% of soil taxa produced new rRNA and therefore were metabolically active.

Original languageEnglish (US)
JournalISME Journal
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Ribosomal RNA
Isotopes
stable isotopes
RNA
stable isotope
Soil
ribosomal RNA
Ribonucleotides
ribonucleotides
soil
new taxon
synthesis
incubation
DNA

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Quantitative stable isotope probing with H2 18O reveals that most bacterial taxa in soil synthesize new ribosomal RNA. / Papp, Katerina; Mau, Rebecca L.; Hayer, Michaela; Koch, Benjamin J.; Hungate, Bruce A.; Schwartz, Egbert.

In: ISME Journal, 01.01.2018.

Research output: Contribution to journalArticle

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