Quantitative histopathology for evaluation of in vivo biocompatibility associated with biomedical implants

Robert B. Diller, Robert G. Audet, Robert S Kellar

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

In the current chapter, digital morphometric analysis (DMA) was used to quantify two markers of biocompatibility around commonly used biomaterials. In the field of biomaterial evaluation for biocompatibility, more sophisticated methods are now being used to precisely characterize the elicited response from the surrounding tissue towards the implanted material. One reason for this is due to the fact that many newer biomaterial innovations are incorporating pharmaceutical agents (e.g., drug eluting stents and drug eluting balloons). Therefore, as described in many of the other chapters in this book, components of toxicology and pharmacology are being evaluated along with biocompatibility. In this chapter, expanded polytetrafluoroethylene (ePTFE) was compared to polypropylene (PP) for inflammatory and foreign body response. Each material was implanted into dorsal subcutaneous spaces and evaluated after 2, 4, and 12 weeks. Each sample was reacted with an antibody to cluster of differentiation-68 (CD-68). The resulting slides were scanned and evaluated using DMA in order to obtain accurate, reproducible, and consistent results. Expanded PTFE demonstrated a lower overall weighted inflammatory score when compared to PP across all timepoints. This chapter describes the use of DMA as a novel approach to measure the inflammatory score that is associated with a specific biomaterial. Current and future medical devices will need to use various analytical tools to comprehensively assess device, biomaterial, or a combination therapy's biocompatibility. The next chapter further describes how quantitative data from histology and immunohistochemistry assessments can be coupled with quantitative polymerase chain reactions (PCR) as assessment tools for product development.

Original languageEnglish (US)
Title of host publicationMolecular Histopathology and Tissue Biomarkers in Drug and Diagnostic Development
PublisherSpringer New York
Pages153-162
Number of pages10
ISBN (Electronic)9781493926817
ISBN (Print)9781493926800
DOIs
StatePublished - Jun 15 2015

Fingerprint

Biocompatible Materials
Polypropylenes
Polytetrafluoroethylene
Equipment and Supplies
Drug-Eluting Stents
Foreign Bodies
Pharmaceutical Preparations
Toxicology
Histology
Immunohistochemistry
Pharmacology
Polymerase Chain Reaction
Antibodies

Keywords

  • Biocompatibility
  • Biomedical devices
  • Biomedical implants
  • Digital morphometric analysis (DMA)
  • Expanded polytetrafluoroethylene (ePTFE)
  • Foreign body response
  • Inflammation
  • Inflammatory score
  • Medical devices
  • Medical implants
  • Polypropylene (PP)
  • Quantitative histopathology

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Diller, R. B., Audet, R. G., & Kellar, R. S. (2015). Quantitative histopathology for evaluation of in vivo biocompatibility associated with biomedical implants. In Molecular Histopathology and Tissue Biomarkers in Drug and Diagnostic Development (pp. 153-162). Springer New York. https://doi.org/10.1007/7653_2014_37

Quantitative histopathology for evaluation of in vivo biocompatibility associated with biomedical implants. / Diller, Robert B.; Audet, Robert G.; Kellar, Robert S.

Molecular Histopathology and Tissue Biomarkers in Drug and Diagnostic Development. Springer New York, 2015. p. 153-162.

Research output: Chapter in Book/Report/Conference proceedingChapter

Diller, RB, Audet, RG & Kellar, RS 2015, Quantitative histopathology for evaluation of in vivo biocompatibility associated with biomedical implants. in Molecular Histopathology and Tissue Biomarkers in Drug and Diagnostic Development. Springer New York, pp. 153-162. https://doi.org/10.1007/7653_2014_37
Diller RB, Audet RG, Kellar RS. Quantitative histopathology for evaluation of in vivo biocompatibility associated with biomedical implants. In Molecular Histopathology and Tissue Biomarkers in Drug and Diagnostic Development. Springer New York. 2015. p. 153-162 https://doi.org/10.1007/7653_2014_37
Diller, Robert B. ; Audet, Robert G. ; Kellar, Robert S. / Quantitative histopathology for evaluation of in vivo biocompatibility associated with biomedical implants. Molecular Histopathology and Tissue Biomarkers in Drug and Diagnostic Development. Springer New York, 2015. pp. 153-162
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