Abstract
We investigated a Q fever outbreak with human patients showing high fever, respiratory tract symptoms, headache and retrosternal pain in southern Hungary in the spring and summer of 2013. Seventy human cases were confirmed by analysing their serum and blood samples with micro-immunofluorescence test and real-time PCR. The source of infection was a merino sheep flock of 450 ewes, in which 44.6% (25/56) seropositivity was detected by enzyme-linked immunosorbent assay. Coxiella burnetii DNA was detected by real-time PCR in the milk of four of 20 individuals and in two thirds (41/65) of the manure samples. The multispacer sequence typing examination of C. burnetii DNA revealed sequence type 18 in one human sample and two manure samples from the sheep flock. The multilocus variable-number tandem repeat analysis pattern of the sheep and human strains were also almost identical, 4/5-9-3-3-0-5 (Ms23-Ms24-Ms27-Ms28-Ms33-Ms34). It is hypothesised that dried manure and maternal fluid contaminated with C. burnetii was dispersed by the wind from the sheep farm towards the local inhabitants. The manure was eliminated in June and the farm was disinfected in July. The outbreak ended at the end of July 2013.
| Original language | English (US) |
|---|---|
| Article number | 20863 |
| Journal | Euro surveillance : bulletin Européen sur les maladies transmissibles = European communicable disease bulletin |
| Volume | 19 |
| Issue number | 30 |
| State | Published - 2015 |
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ASJC Scopus subject areas
- Epidemiology
- Public Health, Environmental and Occupational Health
- Virology
Cite this
Q fever epidemic in Hungary, April to July 2013. / Gyuranecz, M.; Sulyok, K. M.; Balla, E.; Mag, T.; Balázs, A.; Simor, Z.; Dénes, B.; Hornok, S.; Bajnóczi, P.; Hornstra, H. M.; Pearson, Talima R; Keim, Paul S; Dán, A.
In: Euro surveillance : bulletin Européen sur les maladies transmissibles = European communicable disease bulletin, Vol. 19, No. 30, 20863, 2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Q fever epidemic in Hungary, April to July 2013
AU - Gyuranecz, M.
AU - Sulyok, K. M.
AU - Balla, E.
AU - Mag, T.
AU - Balázs, A.
AU - Simor, Z.
AU - Dénes, B.
AU - Hornok, S.
AU - Bajnóczi, P.
AU - Hornstra, H. M.
AU - Pearson, Talima R
AU - Keim, Paul S
AU - Dán, A.
PY - 2015
Y1 - 2015
N2 - We investigated a Q fever outbreak with human patients showing high fever, respiratory tract symptoms, headache and retrosternal pain in southern Hungary in the spring and summer of 2013. Seventy human cases were confirmed by analysing their serum and blood samples with micro-immunofluorescence test and real-time PCR. The source of infection was a merino sheep flock of 450 ewes, in which 44.6% (25/56) seropositivity was detected by enzyme-linked immunosorbent assay. Coxiella burnetii DNA was detected by real-time PCR in the milk of four of 20 individuals and in two thirds (41/65) of the manure samples. The multispacer sequence typing examination of C. burnetii DNA revealed sequence type 18 in one human sample and two manure samples from the sheep flock. The multilocus variable-number tandem repeat analysis pattern of the sheep and human strains were also almost identical, 4/5-9-3-3-0-5 (Ms23-Ms24-Ms27-Ms28-Ms33-Ms34). It is hypothesised that dried manure and maternal fluid contaminated with C. burnetii was dispersed by the wind from the sheep farm towards the local inhabitants. The manure was eliminated in June and the farm was disinfected in July. The outbreak ended at the end of July 2013.
AB - We investigated a Q fever outbreak with human patients showing high fever, respiratory tract symptoms, headache and retrosternal pain in southern Hungary in the spring and summer of 2013. Seventy human cases were confirmed by analysing their serum and blood samples with micro-immunofluorescence test and real-time PCR. The source of infection was a merino sheep flock of 450 ewes, in which 44.6% (25/56) seropositivity was detected by enzyme-linked immunosorbent assay. Coxiella burnetii DNA was detected by real-time PCR in the milk of four of 20 individuals and in two thirds (41/65) of the manure samples. The multispacer sequence typing examination of C. burnetii DNA revealed sequence type 18 in one human sample and two manure samples from the sheep flock. The multilocus variable-number tandem repeat analysis pattern of the sheep and human strains were also almost identical, 4/5-9-3-3-0-5 (Ms23-Ms24-Ms27-Ms28-Ms33-Ms34). It is hypothesised that dried manure and maternal fluid contaminated with C. burnetii was dispersed by the wind from the sheep farm towards the local inhabitants. The manure was eliminated in June and the farm was disinfected in July. The outbreak ended at the end of July 2013.
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UR - http://www.scopus.com/inward/citedby.url?scp=84928248369&partnerID=8YFLogxK
M3 - Article
C2 - 25108535
AN - SCOPUS:84928248369
VL - 19
JO - Eurosurveillance
JF - Eurosurveillance
SN - 1560-7917
IS - 30
M1 - 20863
ER -