Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells

B. K. Haarer, S. H. Lillie, Alison Adams, V. Magdolen, W. Bandlow, S. S. Brown

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Müller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins from other organisms in molecular mass and in the ability to bind to polyproline, retard the rate of actin polymerization, and inhibit hydrolysis of ATP by monomeric actin. Using strains that carry disruptions or deletions of the profilin gene, we have found that, under appropriate conditions, cells can survive without detectable profilin. Such cells grow slowly, are temperature sensitive, lose the normal ellipsoidal shape of yeast cells, often become multinucleate, and generally grow much larger than wild-type cells. In addition, these cells exhibit delocalized deposition of cell wall chitin and have dramatically altered actin distributions.

Original languageEnglish (US)
Pages (from-to)105-114
Number of pages10
JournalJournal of Cell Biology
Volume110
Issue number1
StatePublished - Jan 1990
Externally publishedYes

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Profilins
Saccharomyces cerevisiae
Yeasts
Actins
Chitin
Cell Shape
Gene Deletion
Polymerization
Cell Wall
Amino Acid Sequence
Hydrolysis
Temperature
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Haarer, B. K., Lillie, S. H., Adams, A., Magdolen, V., Bandlow, W., & Brown, S. S. (1990). Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells. Journal of Cell Biology, 110(1), 105-114.

Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells. / Haarer, B. K.; Lillie, S. H.; Adams, Alison; Magdolen, V.; Bandlow, W.; Brown, S. S.

In: Journal of Cell Biology, Vol. 110, No. 1, 01.1990, p. 105-114.

Research output: Contribution to journalArticle

Haarer, BK, Lillie, SH, Adams, A, Magdolen, V, Bandlow, W & Brown, SS 1990, 'Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells', Journal of Cell Biology, vol. 110, no. 1, pp. 105-114.
Haarer, B. K. ; Lillie, S. H. ; Adams, Alison ; Magdolen, V. ; Bandlow, W. ; Brown, S. S. / Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells. In: Journal of Cell Biology. 1990 ; Vol. 110, No. 1. pp. 105-114.
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