Proteomic analysis of four Clostridium botulinum strains identifies proteins that link biological responses to proteomic signatures

Brooke L.Deatherage Kaiser, Karen K. Hill, Theresa J. Smith, Charles H.D. Williamson, Paul S Keim, Jason W. Sahl, Karen L. Wahl

Research output: Contribution to journalArticle

Abstract

Microorganisms alter gene and protein expression in response to environmental conditions to adapt and survive. Whereas the genetic composition of a microbe represents an organism's biological potential, the proteins expressed provide a functional readout of the organism's response to the environment. Understanding protein expression patterns in response to specific environmental conditions furthers fundamental knowledge about a microbe, which can be especially useful for understudied organisms such as Clostridium botulinum examined herein. In addition, protein expression patterns that reproducibly occur in certain growth conditions hold potential in fields such as microbial forensics, in which determination of conditions in which an unknown possible biothreat sample had been grown may be important. To investigate the identity and reproducibility of protein profile patterns for varied strains, we defined the proteomic profiles of four Group I strains of Clostridium botulinum, a Category A biothreat agent and the organism responsible for the production of the botulinum neurotoxin (BoNT), in two different culture media grown for five days. The four C. botulinum strains produced one of three neurotoxins (BoNT/A, /B, or /F), and their protein profiles were compared to that of a fifth non-Toxigenic strain of C. sporogenes. These strains each had DNA sequences available to assist in accurate protein identification. Differing culture growth phase, bacterial strain, and growth medium resulted in reproducible protein profiles, which were used to calculate relative protein abundance ratios as an internally normalized metric of microbial growth in varying conditions. The resulting protein profiles provide functional information about how four Group I C. botulinum strains and a C. sporogenes strain respond to the culture environment during growth and explores the feasibility of using these proteins to characterize unknown samples.

Original languageEnglish (US)
Article numbere0205586
JournalPLoS One
Volume13
Issue number10
DOIs
StatePublished - Oct 1 2018

Fingerprint

Clostridium botulinum
Clostridium
Proteomics
proteomics
Proteins
proteins
botulinum toxin
protein synthesis
organisms
microorganisms
Growth
Neurotoxins
culture media
nontoxigenic strains
environmental factors
neurotoxins
link protein
reproducibility
Type A Botulinum Toxins
microbial growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Proteomic analysis of four Clostridium botulinum strains identifies proteins that link biological responses to proteomic signatures. / Kaiser, Brooke L.Deatherage; Hill, Karen K.; Smith, Theresa J.; Williamson, Charles H.D.; Keim, Paul S; Sahl, Jason W.; Wahl, Karen L.

In: PLoS One, Vol. 13, No. 10, e0205586, 01.10.2018.

Research output: Contribution to journalArticle

Kaiser, Brooke L.Deatherage ; Hill, Karen K. ; Smith, Theresa J. ; Williamson, Charles H.D. ; Keim, Paul S ; Sahl, Jason W. ; Wahl, Karen L. / Proteomic analysis of four Clostridium botulinum strains identifies proteins that link biological responses to proteomic signatures. In: PLoS One. 2018 ; Vol. 13, No. 10.
@article{07c7cf04a98e44d48e0c6f18ebee9ac0,
title = "Proteomic analysis of four Clostridium botulinum strains identifies proteins that link biological responses to proteomic signatures",
abstract = "Microorganisms alter gene and protein expression in response to environmental conditions to adapt and survive. Whereas the genetic composition of a microbe represents an organism's biological potential, the proteins expressed provide a functional readout of the organism's response to the environment. Understanding protein expression patterns in response to specific environmental conditions furthers fundamental knowledge about a microbe, which can be especially useful for understudied organisms such as Clostridium botulinum examined herein. In addition, protein expression patterns that reproducibly occur in certain growth conditions hold potential in fields such as microbial forensics, in which determination of conditions in which an unknown possible biothreat sample had been grown may be important. To investigate the identity and reproducibility of protein profile patterns for varied strains, we defined the proteomic profiles of four Group I strains of Clostridium botulinum, a Category A biothreat agent and the organism responsible for the production of the botulinum neurotoxin (BoNT), in two different culture media grown for five days. The four C. botulinum strains produced one of three neurotoxins (BoNT/A, /B, or /F), and their protein profiles were compared to that of a fifth non-Toxigenic strain of C. sporogenes. These strains each had DNA sequences available to assist in accurate protein identification. Differing culture growth phase, bacterial strain, and growth medium resulted in reproducible protein profiles, which were used to calculate relative protein abundance ratios as an internally normalized metric of microbial growth in varying conditions. The resulting protein profiles provide functional information about how four Group I C. botulinum strains and a C. sporogenes strain respond to the culture environment during growth and explores the feasibility of using these proteins to characterize unknown samples.",
author = "Kaiser, {Brooke L.Deatherage} and Hill, {Karen K.} and Smith, {Theresa J.} and Williamson, {Charles H.D.} and Keim, {Paul S} and Sahl, {Jason W.} and Wahl, {Karen L.}",
year = "2018",
month = "10",
day = "1",
doi = "10.1371/journal.pone.0205586",
language = "English (US)",
volume = "13",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

TY - JOUR

T1 - Proteomic analysis of four Clostridium botulinum strains identifies proteins that link biological responses to proteomic signatures

AU - Kaiser, Brooke L.Deatherage

AU - Hill, Karen K.

AU - Smith, Theresa J.

AU - Williamson, Charles H.D.

AU - Keim, Paul S

AU - Sahl, Jason W.

AU - Wahl, Karen L.

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Microorganisms alter gene and protein expression in response to environmental conditions to adapt and survive. Whereas the genetic composition of a microbe represents an organism's biological potential, the proteins expressed provide a functional readout of the organism's response to the environment. Understanding protein expression patterns in response to specific environmental conditions furthers fundamental knowledge about a microbe, which can be especially useful for understudied organisms such as Clostridium botulinum examined herein. In addition, protein expression patterns that reproducibly occur in certain growth conditions hold potential in fields such as microbial forensics, in which determination of conditions in which an unknown possible biothreat sample had been grown may be important. To investigate the identity and reproducibility of protein profile patterns for varied strains, we defined the proteomic profiles of four Group I strains of Clostridium botulinum, a Category A biothreat agent and the organism responsible for the production of the botulinum neurotoxin (BoNT), in two different culture media grown for five days. The four C. botulinum strains produced one of three neurotoxins (BoNT/A, /B, or /F), and their protein profiles were compared to that of a fifth non-Toxigenic strain of C. sporogenes. These strains each had DNA sequences available to assist in accurate protein identification. Differing culture growth phase, bacterial strain, and growth medium resulted in reproducible protein profiles, which were used to calculate relative protein abundance ratios as an internally normalized metric of microbial growth in varying conditions. The resulting protein profiles provide functional information about how four Group I C. botulinum strains and a C. sporogenes strain respond to the culture environment during growth and explores the feasibility of using these proteins to characterize unknown samples.

AB - Microorganisms alter gene and protein expression in response to environmental conditions to adapt and survive. Whereas the genetic composition of a microbe represents an organism's biological potential, the proteins expressed provide a functional readout of the organism's response to the environment. Understanding protein expression patterns in response to specific environmental conditions furthers fundamental knowledge about a microbe, which can be especially useful for understudied organisms such as Clostridium botulinum examined herein. In addition, protein expression patterns that reproducibly occur in certain growth conditions hold potential in fields such as microbial forensics, in which determination of conditions in which an unknown possible biothreat sample had been grown may be important. To investigate the identity and reproducibility of protein profile patterns for varied strains, we defined the proteomic profiles of four Group I strains of Clostridium botulinum, a Category A biothreat agent and the organism responsible for the production of the botulinum neurotoxin (BoNT), in two different culture media grown for five days. The four C. botulinum strains produced one of three neurotoxins (BoNT/A, /B, or /F), and their protein profiles were compared to that of a fifth non-Toxigenic strain of C. sporogenes. These strains each had DNA sequences available to assist in accurate protein identification. Differing culture growth phase, bacterial strain, and growth medium resulted in reproducible protein profiles, which were used to calculate relative protein abundance ratios as an internally normalized metric of microbial growth in varying conditions. The resulting protein profiles provide functional information about how four Group I C. botulinum strains and a C. sporogenes strain respond to the culture environment during growth and explores the feasibility of using these proteins to characterize unknown samples.

UR - http://www.scopus.com/inward/record.url?scp=85055073018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055073018&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0205586

DO - 10.1371/journal.pone.0205586

M3 - Article

VL - 13

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 10

M1 - e0205586

ER -