Proteomic analysis of Bacillus anthracis Sterne vegetative cells

Anthony W. Francis, Christy E. Ruggiero, Andrew T. Koppisch, Jingao Dong, Jian Song, Thomas Brettin, Srinivas Iyer

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Mass spectrometry and proteomics have found increasing use as tools for the rapid detection of pathogenic bacteria, even when they are in a mixture of non-pathogenic relatives. The success of this technique is greatly augmented by the availability of publicly accessible proteomic databases for specific pathogenic bacteria. To aid proteomic detection analyses for the causative agent of anthrax, we have constructed a comprehensive proteomic catalogue of vegetative Bacillus anthracis Sterne cells using liquid chromatography tandem-mass spectrometry. Proteins were separated by molecular weight or isoelectric point prior to tryptic digestion. Alternatively, the whole protein extract was digested and tryptic peptides were separated by cation exchange chromatography prior to Reverse Phase-LC-MS/MS. The use of three complementary, pre-analytical separation techniques resulted in the identification of 1048 unique proteins, including 694 cytosolic, 153 membrane (including 27 cell wall), and 30 secreted proteins, accounting for 19% of the total predicted proteome. Each identified protein was functionally categorized using the gene attribute database from TIGR CMR. These results provide a large proteomic catalogue of vegetative B. anthracis cells and, coupled with the recent proteomic catalogue of B. anthracis spore proteins, form a thorough summary of proteins expressed in the active and dormant stages of this organism.

Original languageEnglish (US)
Pages (from-to)191-200
Number of pages10
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1748
Issue number2
DOIs
StatePublished - May 15 2005

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Keywords

  • Bacillus anthracis Sterne
  • Mass spectrometry
  • Proteomics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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