Proteolytic enzymes in excretory-secretory products from adult Nema tospiroides dubius

F. G. Monroy, C. J R Cayzer, J. H. Adams, C. Dobson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Proteolytic enzymes in excretory-secretory products from adult Nematospiroides dubius. International Journal for Parasitology 19: 129-131. Excretory-secretory products (ES), collected from in vitro cultures of adult Nematospiroides dubius, were examined for proteolytic enzyme activity. ES enzymes had a pH optimum of 8.0 and their activity was sensitive to serine-proteinase inhibitors. Three SDS-resistant proteases were identified in ES at molecular weight (mol. wt) 200,000, 105,000 and 48,000 by incorporating substrates into the matrices of sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) gels.

Original languageEnglish (US)
Pages (from-to)129-131
Number of pages3
JournalInternational Journal for Parasitology
Volume19
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Tetrachloroethylene
Nematospiroides dubius
Peptide Hydrolases
Parasitology
Serine Proteinase Inhibitors
Sodium Dodecyl Sulfate
Electrophoresis
Polyacrylamide Gel Electrophoresis
Molecular Weight
Gels
Enzymes

Keywords

  • enzymes
  • excretory-secretory products
  • inhibitors
  • Nematoda
  • Nematospiroides dubius

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Proteolytic enzymes in excretory-secretory products from adult Nema tospiroides dubius. / Monroy, F. G.; Cayzer, C. J R; Adams, J. H.; Dobson, C.

In: International Journal for Parasitology, Vol. 19, No. 1, 1989, p. 129-131.

Research output: Contribution to journalArticle

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