PrimerProspector: De novo design and taxonomic analysis of barcoded polymerase chain reaction primers

William A. Walters, James G Caporaso, Christian L. Lauber, Donna Berg-Lyons, Noah Fierer, Rob Knight

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

Motivation: PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task. Results: PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.

Original languageEnglish (US)
Article numberbtr087
Pages (from-to)1159-1161
Number of pages3
JournalBioinformatics
Volume27
Issue number8
DOIs
StatePublished - Apr 2011
Externally publishedYes

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Polymerase Chain Reaction
Open Source Software
Polymerase chain reaction
Software Package
Software packages
Amplification
Sequencing
High Throughput
DNA
Software
Research Personnel
Throughput
Technology
Evaluate
Context
Design
Open source software

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computational Theory and Mathematics
  • Computer Science Applications
  • Computational Mathematics
  • Statistics and Probability
  • Medicine(all)

Cite this

PrimerProspector : De novo design and taxonomic analysis of barcoded polymerase chain reaction primers. / Walters, William A.; Caporaso, James G; Lauber, Christian L.; Berg-Lyons, Donna; Fierer, Noah; Knight, Rob.

In: Bioinformatics, Vol. 27, No. 8, btr087, 04.2011, p. 1159-1161.

Research output: Contribution to journalArticle

Walters, William A. ; Caporaso, James G ; Lauber, Christian L. ; Berg-Lyons, Donna ; Fierer, Noah ; Knight, Rob. / PrimerProspector : De novo design and taxonomic analysis of barcoded polymerase chain reaction primers. In: Bioinformatics. 2011 ; Vol. 27, No. 8. pp. 1159-1161.
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