PrimerProspector: De novo design and taxonomic analysis of barcoded polymerase chain reaction primers

William A. Walters, J. Gregory Caporaso, Christian L. Lauber, Donna Berg-Lyons, Noah Fierer, Rob Knight

Research output: Contribution to journalArticle

199 Scopus citations

Abstract

Motivation: PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task. Results: PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.

Original languageEnglish (US)
Article numberbtr087
Pages (from-to)1159-1161
Number of pages3
JournalBioinformatics
Volume27
Issue number8
DOIs
StatePublished - Apr 2011

ASJC Scopus subject areas

  • Statistics and Probability
  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Computational Theory and Mathematics
  • Computational Mathematics

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