Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation

Jan Sunner, Recep Avci, Lee Richards, Gary Groenewold, Jani Ingram, Megan Arthun

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-μm IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.

Original languageEnglish (US)
Pages (from-to)285-287
Number of pages3
JournalJournal of Microbiological Methods
Volume54
Issue number2
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Keywords

  • Dehydration
  • Electron microscopy
  • Fixation
  • IR
  • Imaging
  • Infrared laser
  • Morphology
  • SEM
  • Yeast cells

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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