Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation

Jan Sunner, Recep Avci, Lee Richards, Gary Groenewold, Jani C Ingram, Megan Arthun

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-μm IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.

Original languageEnglish (US)
Pages (from-to)285-287
Number of pages3
JournalJournal of Microbiological Methods
Volume54
Issue number2
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Fingerprint

Electron Scanning Microscopy
Lasers
Yeasts
Saccharomyces cerevisiae
Air

Keywords

  • Dehydration
  • Electron microscopy
  • Fixation
  • Imaging
  • Infrared laser
  • IR
  • Morphology
  • SEM
  • Yeast cells

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation. / Sunner, Jan; Avci, Recep; Richards, Lee; Groenewold, Gary; Ingram, Jani C; Arthun, Megan.

In: Journal of Microbiological Methods, Vol. 54, No. 2, 01.08.2003, p. 285-287.

Research output: Contribution to journalArticle

Sunner, Jan ; Avci, Recep ; Richards, Lee ; Groenewold, Gary ; Ingram, Jani C ; Arthun, Megan. / Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation. In: Journal of Microbiological Methods. 2003 ; Vol. 54, No. 2. pp. 285-287.
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