Presence and Diversity of Streptomyces in Dendroctonus and Sympatric Bark Beetle Galleries Across North America

Jiri Hulcr, Aaron S. Adams, Kenneth Raffa, Richard Hofstetter, Kier D. Klepzig, Cameron R. Currie

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Recent studies have revealed several examples of intimate associations between insects and Actinobacteria, including the Southern Pine Beetle Dendroctonus frontalis and the Spruce Beetle Dendroctonus rufipennis. Here, we surveyed Streptomyces Actinobacteria co-occurring with 10 species of Dendroctonus bark beetles across the United States, using both phylogenetic and community ecology approaches. From these 10 species, and 19 other scolytine beetles that occur in the same trees, we obtained 154 Streptomyces-like isolates and generated 16S sequences from 134 of those. Confirmed 16S sequences of Streptomyces were binned into 36 distinct strains using a threshold of 0.2% sequence divergence. The 16S rDNA phylogeny of all isolates does not correlate with the distribution of strains among beetle species, localities, or parts of the beetles or their galleries. However, we identified three Streptomyces strains occurring repeatedly on Dendroctonus beetles and in their galleries. Identity of these isolates was corroborated using a house-keeping gene sequence (efTu). These strains are not confined to a certain species of beetle, locality, or part of the beetle or their galleries. However, their role as residents in the woodboring insect niche is supported by the repeated association of their 16S and efTu from across the continent, and also having been reported in studies of other subcortical insects.

Original languageEnglish (US)
Pages (from-to)759-768
Number of pages10
JournalMicrobial Ecology
Volume61
Issue number4
DOIs
StatePublished - May 2011

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Dendroctonus
Streptomyces
bark beetles
bark
beetle
Coleoptera
Dendroctonus rufipennis
Dendroctonus frontalis
Actinobacteria
insects
insect
community ecology
phylogeny
North America
niches
nucleotide sequences
niche
divergence
phylogenetics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

Cite this

Presence and Diversity of Streptomyces in Dendroctonus and Sympatric Bark Beetle Galleries Across North America. / Hulcr, Jiri; Adams, Aaron S.; Raffa, Kenneth; Hofstetter, Richard; Klepzig, Kier D.; Currie, Cameron R.

In: Microbial Ecology, Vol. 61, No. 4, 05.2011, p. 759-768.

Research output: Contribution to journalArticle

Hulcr, Jiri ; Adams, Aaron S. ; Raffa, Kenneth ; Hofstetter, Richard ; Klepzig, Kier D. ; Currie, Cameron R. / Presence and Diversity of Streptomyces in Dendroctonus and Sympatric Bark Beetle Galleries Across North America. In: Microbial Ecology. 2011 ; Vol. 61, No. 4. pp. 759-768.
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