Preliminary evidence that age and sex affect exercise-induced hTERT expression

Travis G. Cluckey, Nathan C Nieto, Bridger M. Rodoni, Tinna Traustadottir

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The ability to repair cellular damage is reduced with aging, resulting in cellular senescence. Telomeres shorten as cells divide but the rate of telomere attrition is modulated by telomerase, an enzyme that adds nucleotides to the chromosome. Shelterin is a protein complex that acts as a negative regulator of telomerase. The aim of the present study was to investigate age-related differences in telomerase and shelterin responses to acute exercise. We hypothesized that acute exercise would stimulate an increased activity of telomerase (measured by telomerase reverse transcriptase, hTERT) without an increase in activity of shelterin (measured by telomeric repeat binding factor 2, TRF2) in both young and older individuals and that hTERT response would be attenuated in older individuals. Young (22 ± 2 y, n = 11) and older (60 ± 2 y, n = 8) men and women performed 30 min of cycling. Blood was collected pre-exercise and 30, 60, and 90-min post-exercise. The trial induced a significant hTERT response in the cohort as a whole (p < 0.05) with greater increases in the young as compared to the older group (time-by-group interaction p < 0.05). As expected, TRF2 did not change in response to the trial, however older individuals had a higher TRF2 response at 60 min (p < 0.05). There was an unexpected sex difference, regardless of age, where men had significantly greater hTERT and TRF2 responses to the acute exercise as compared to women (p < 0.05). These data support the hypothesis that aging is associated with attenuated telomerase activation in response to high-intensity exercise; however, this was only evident in men.

Original languageEnglish (US)
Pages (from-to)7-11
Number of pages5
JournalExperimental Gerontology
Volume96
DOIs
StatePublished - Oct 1 2017

Fingerprint

Telomerase
Exercise
Aging of materials
Telomere Shortening
Cell Aging
Telomere
Chromosomes
Sex Characteristics
Repair
Blood
Nucleotides
Chemical activation
Enzymes
Proteins

Keywords

  • Aging
  • hTERT
  • IL-6
  • Sex differences
  • Telomerase
  • TRF2

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Preliminary evidence that age and sex affect exercise-induced hTERT expression. / Cluckey, Travis G.; Nieto, Nathan C; Rodoni, Bridger M.; Traustadottir, Tinna.

In: Experimental Gerontology, Vol. 96, 01.10.2017, p. 7-11.

Research output: Contribution to journalArticle

@article{52637f900a194cb68f374f5db502ff97,
title = "Preliminary evidence that age and sex affect exercise-induced hTERT expression",
abstract = "The ability to repair cellular damage is reduced with aging, resulting in cellular senescence. Telomeres shorten as cells divide but the rate of telomere attrition is modulated by telomerase, an enzyme that adds nucleotides to the chromosome. Shelterin is a protein complex that acts as a negative regulator of telomerase. The aim of the present study was to investigate age-related differences in telomerase and shelterin responses to acute exercise. We hypothesized that acute exercise would stimulate an increased activity of telomerase (measured by telomerase reverse transcriptase, hTERT) without an increase in activity of shelterin (measured by telomeric repeat binding factor 2, TRF2) in both young and older individuals and that hTERT response would be attenuated in older individuals. Young (22 ± 2 y, n = 11) and older (60 ± 2 y, n = 8) men and women performed 30 min of cycling. Blood was collected pre-exercise and 30, 60, and 90-min post-exercise. The trial induced a significant hTERT response in the cohort as a whole (p < 0.05) with greater increases in the young as compared to the older group (time-by-group interaction p < 0.05). As expected, TRF2 did not change in response to the trial, however older individuals had a higher TRF2 response at 60 min (p < 0.05). There was an unexpected sex difference, regardless of age, where men had significantly greater hTERT and TRF2 responses to the acute exercise as compared to women (p < 0.05). These data support the hypothesis that aging is associated with attenuated telomerase activation in response to high-intensity exercise; however, this was only evident in men.",
keywords = "Aging, hTERT, IL-6, Sex differences, Telomerase, TRF2",
author = "Cluckey, {Travis G.} and Nieto, {Nathan C} and Rodoni, {Bridger M.} and Tinna Traustadottir",
year = "2017",
month = "10",
day = "1",
doi = "10.1016/j.exger.2017.06.003",
language = "English (US)",
volume = "96",
pages = "7--11",
journal = "Experimental Gerontology",
issn = "0531-5565",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Preliminary evidence that age and sex affect exercise-induced hTERT expression

AU - Cluckey, Travis G.

AU - Nieto, Nathan C

AU - Rodoni, Bridger M.

AU - Traustadottir, Tinna

PY - 2017/10/1

Y1 - 2017/10/1

N2 - The ability to repair cellular damage is reduced with aging, resulting in cellular senescence. Telomeres shorten as cells divide but the rate of telomere attrition is modulated by telomerase, an enzyme that adds nucleotides to the chromosome. Shelterin is a protein complex that acts as a negative regulator of telomerase. The aim of the present study was to investigate age-related differences in telomerase and shelterin responses to acute exercise. We hypothesized that acute exercise would stimulate an increased activity of telomerase (measured by telomerase reverse transcriptase, hTERT) without an increase in activity of shelterin (measured by telomeric repeat binding factor 2, TRF2) in both young and older individuals and that hTERT response would be attenuated in older individuals. Young (22 ± 2 y, n = 11) and older (60 ± 2 y, n = 8) men and women performed 30 min of cycling. Blood was collected pre-exercise and 30, 60, and 90-min post-exercise. The trial induced a significant hTERT response in the cohort as a whole (p < 0.05) with greater increases in the young as compared to the older group (time-by-group interaction p < 0.05). As expected, TRF2 did not change in response to the trial, however older individuals had a higher TRF2 response at 60 min (p < 0.05). There was an unexpected sex difference, regardless of age, where men had significantly greater hTERT and TRF2 responses to the acute exercise as compared to women (p < 0.05). These data support the hypothesis that aging is associated with attenuated telomerase activation in response to high-intensity exercise; however, this was only evident in men.

AB - The ability to repair cellular damage is reduced with aging, resulting in cellular senescence. Telomeres shorten as cells divide but the rate of telomere attrition is modulated by telomerase, an enzyme that adds nucleotides to the chromosome. Shelterin is a protein complex that acts as a negative regulator of telomerase. The aim of the present study was to investigate age-related differences in telomerase and shelterin responses to acute exercise. We hypothesized that acute exercise would stimulate an increased activity of telomerase (measured by telomerase reverse transcriptase, hTERT) without an increase in activity of shelterin (measured by telomeric repeat binding factor 2, TRF2) in both young and older individuals and that hTERT response would be attenuated in older individuals. Young (22 ± 2 y, n = 11) and older (60 ± 2 y, n = 8) men and women performed 30 min of cycling. Blood was collected pre-exercise and 30, 60, and 90-min post-exercise. The trial induced a significant hTERT response in the cohort as a whole (p < 0.05) with greater increases in the young as compared to the older group (time-by-group interaction p < 0.05). As expected, TRF2 did not change in response to the trial, however older individuals had a higher TRF2 response at 60 min (p < 0.05). There was an unexpected sex difference, regardless of age, where men had significantly greater hTERT and TRF2 responses to the acute exercise as compared to women (p < 0.05). These data support the hypothesis that aging is associated with attenuated telomerase activation in response to high-intensity exercise; however, this was only evident in men.

KW - Aging

KW - hTERT

KW - IL-6

KW - Sex differences

KW - Telomerase

KW - TRF2

UR - http://www.scopus.com/inward/record.url?scp=85020240117&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020240117&partnerID=8YFLogxK

U2 - 10.1016/j.exger.2017.06.003

DO - 10.1016/j.exger.2017.06.003

M3 - Article

C2 - 28587932

AN - SCOPUS:85020240117

VL - 96

SP - 7

EP - 11

JO - Experimental Gerontology

JF - Experimental Gerontology

SN - 0531-5565

ER -