Potential role of Thermus thermophilus and T. oshimai in high rates of nitrous oxide (N 2O) production in ~80°C hot springs in the US Great Basin

B. P. Hedlund, A. I. McDonald, J. Lam, J. A. Dodsworth, J. R. Brown, Bruce A Hungate

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Ambient nitrous oxide (N 2O) emissions from Great Boiling Spring (GBS) in the US Great Basin depended on temperature, with the highest flux, 67.8±2.6μmol N 2O-Nm -2day -1, occurring in the large source pool at 82°C. This rate of N 2O production contrasted with negligible production from nearby soils and was similar to rates from soils and sediments impacted with agricultural fertilizers. To investigate the source of N 2O, a variety of approaches were used to enrich and isolate heterotrophic micro-organisms, and isolates were screened for nitrate reduction ability. Nitrate-respiring isolates were identified by 16S rRNA gene sequencing as Thermus thermophilus (31 isolates) and T. oshimai (three isolates). All isolates reduced nitrate to N 2O but not to dinitrogen and were unable to grow with N 2O as a terminal electron acceptor. Representative T. thermophilus and T. oshimai strains contained genes with 96-98% and 93% DNA identity, respectively, to the nitrate reductase catalytic subunit gene (narG) of T. thermophilus HB8. These data implicate T. thermophilus and T. oshimai in high flux of N 2O in GBS and raise questions about the genetic basis of the incomplete denitrification pathway in these organisms and on the fate of biogenic N 2O in geothermal environments.

Original languageEnglish (US)
Pages (from-to)471-480
Number of pages10
JournalGeobiology
Volume9
Issue number6
DOIs
StatePublished - Nov 2011

Fingerprint

Thermus oshimai
Thermus thermophilus
hot springs
thermal spring
nitrous oxide
basins
nitrate
boiling
basin
gene
nitrates
genes
nitrate reduction
protein subunits
nitrate reductase
denitrification
soil
electrons
fertilizer
ribosomal RNA

ASJC Scopus subject areas

  • Environmental Science(all)
  • Ecology, Evolution, Behavior and Systematics
  • Earth and Planetary Sciences(all)

Cite this

Potential role of Thermus thermophilus and T. oshimai in high rates of nitrous oxide (N 2O) production in ~80°C hot springs in the US Great Basin. / Hedlund, B. P.; McDonald, A. I.; Lam, J.; Dodsworth, J. A.; Brown, J. R.; Hungate, Bruce A.

In: Geobiology, Vol. 9, No. 6, 11.2011, p. 471-480.

Research output: Contribution to journalArticle

Hedlund, B. P. ; McDonald, A. I. ; Lam, J. ; Dodsworth, J. A. ; Brown, J. R. ; Hungate, Bruce A. / Potential role of Thermus thermophilus and T. oshimai in high rates of nitrous oxide (N 2O) production in ~80°C hot springs in the US Great Basin. In: Geobiology. 2011 ; Vol. 9, No. 6. pp. 471-480.
@article{d4e43a40fa224f36a36dc27bf8cb49a9,
title = "Potential role of Thermus thermophilus and T. oshimai in high rates of nitrous oxide (N 2O) production in ~80°C hot springs in the US Great Basin",
abstract = "Ambient nitrous oxide (N 2O) emissions from Great Boiling Spring (GBS) in the US Great Basin depended on temperature, with the highest flux, 67.8±2.6μmol N 2O-Nm -2day -1, occurring in the large source pool at 82°C. This rate of N 2O production contrasted with negligible production from nearby soils and was similar to rates from soils and sediments impacted with agricultural fertilizers. To investigate the source of N 2O, a variety of approaches were used to enrich and isolate heterotrophic micro-organisms, and isolates were screened for nitrate reduction ability. Nitrate-respiring isolates were identified by 16S rRNA gene sequencing as Thermus thermophilus (31 isolates) and T. oshimai (three isolates). All isolates reduced nitrate to N 2O but not to dinitrogen and were unable to grow with N 2O as a terminal electron acceptor. Representative T. thermophilus and T. oshimai strains contained genes with 96-98{\%} and 93{\%} DNA identity, respectively, to the nitrate reductase catalytic subunit gene (narG) of T. thermophilus HB8. These data implicate T. thermophilus and T. oshimai in high flux of N 2O in GBS and raise questions about the genetic basis of the incomplete denitrification pathway in these organisms and on the fate of biogenic N 2O in geothermal environments.",
author = "Hedlund, {B. P.} and McDonald, {A. I.} and J. Lam and Dodsworth, {J. A.} and Brown, {J. R.} and Hungate, {Bruce A}",
year = "2011",
month = "11",
doi = "10.1111/j.1472-4669.2011.00295.x",
language = "English (US)",
volume = "9",
pages = "471--480",
journal = "Geobiology",
issn = "1472-4677",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Potential role of Thermus thermophilus and T. oshimai in high rates of nitrous oxide (N 2O) production in ~80°C hot springs in the US Great Basin

AU - Hedlund, B. P.

AU - McDonald, A. I.

AU - Lam, J.

AU - Dodsworth, J. A.

AU - Brown, J. R.

AU - Hungate, Bruce A

PY - 2011/11

Y1 - 2011/11

N2 - Ambient nitrous oxide (N 2O) emissions from Great Boiling Spring (GBS) in the US Great Basin depended on temperature, with the highest flux, 67.8±2.6μmol N 2O-Nm -2day -1, occurring in the large source pool at 82°C. This rate of N 2O production contrasted with negligible production from nearby soils and was similar to rates from soils and sediments impacted with agricultural fertilizers. To investigate the source of N 2O, a variety of approaches were used to enrich and isolate heterotrophic micro-organisms, and isolates were screened for nitrate reduction ability. Nitrate-respiring isolates were identified by 16S rRNA gene sequencing as Thermus thermophilus (31 isolates) and T. oshimai (three isolates). All isolates reduced nitrate to N 2O but not to dinitrogen and were unable to grow with N 2O as a terminal electron acceptor. Representative T. thermophilus and T. oshimai strains contained genes with 96-98% and 93% DNA identity, respectively, to the nitrate reductase catalytic subunit gene (narG) of T. thermophilus HB8. These data implicate T. thermophilus and T. oshimai in high flux of N 2O in GBS and raise questions about the genetic basis of the incomplete denitrification pathway in these organisms and on the fate of biogenic N 2O in geothermal environments.

AB - Ambient nitrous oxide (N 2O) emissions from Great Boiling Spring (GBS) in the US Great Basin depended on temperature, with the highest flux, 67.8±2.6μmol N 2O-Nm -2day -1, occurring in the large source pool at 82°C. This rate of N 2O production contrasted with negligible production from nearby soils and was similar to rates from soils and sediments impacted with agricultural fertilizers. To investigate the source of N 2O, a variety of approaches were used to enrich and isolate heterotrophic micro-organisms, and isolates were screened for nitrate reduction ability. Nitrate-respiring isolates were identified by 16S rRNA gene sequencing as Thermus thermophilus (31 isolates) and T. oshimai (three isolates). All isolates reduced nitrate to N 2O but not to dinitrogen and were unable to grow with N 2O as a terminal electron acceptor. Representative T. thermophilus and T. oshimai strains contained genes with 96-98% and 93% DNA identity, respectively, to the nitrate reductase catalytic subunit gene (narG) of T. thermophilus HB8. These data implicate T. thermophilus and T. oshimai in high flux of N 2O in GBS and raise questions about the genetic basis of the incomplete denitrification pathway in these organisms and on the fate of biogenic N 2O in geothermal environments.

UR - http://www.scopus.com/inward/record.url?scp=80054060616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054060616&partnerID=8YFLogxK

U2 - 10.1111/j.1472-4669.2011.00295.x

DO - 10.1111/j.1472-4669.2011.00295.x

M3 - Article

VL - 9

SP - 471

EP - 480

JO - Geobiology

JF - Geobiology

SN - 1472-4677

IS - 6

ER -