Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation

Andrew T Koppisch, Cindy C Browder, Amanda L. Moe, Jacob T. Shelley, Blaine A. Kinkel, Larry E. Hersman, Srinivas Iyer, Christy E. Ruggiero

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.

Original languageEnglish (US)
Pages (from-to)577-585
Number of pages9
JournalBioMetals
Volume18
Issue number6
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Bacillus anthracis
Siderophores
siderophores
Bacilli
Starvation
starvation
Iron
Pathogens
iron
Size exclusion chromatography
Ultraviolet spectroscopy
Structural analysis
Nuclear magnetic resonance spectroscopy
Marinobacter hydrocarbonoclasticus
Marinobacter
Resins
pathogens
Bacillus
resins
Gel Chromatography

Keywords

  • Bacillibactin
  • Bacillus anthracis
  • Iron
  • Petrobactin
  • Siderophores

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation. / Koppisch, Andrew T; Browder, Cindy C; Moe, Amanda L.; Shelley, Jacob T.; Kinkel, Blaine A.; Hersman, Larry E.; Iyer, Srinivas; Ruggiero, Christy E.

In: BioMetals, Vol. 18, No. 6, 12.2005, p. 577-585.

Research output: Contribution to journalArticle

Koppisch, Andrew T ; Browder, Cindy C ; Moe, Amanda L. ; Shelley, Jacob T. ; Kinkel, Blaine A. ; Hersman, Larry E. ; Iyer, Srinivas ; Ruggiero, Christy E. / Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation. In: BioMetals. 2005 ; Vol. 18, No. 6. pp. 577-585.
@article{537a8537336a4f2b91f4008d8919df17,
title = "Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation",
abstract = "The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.",
keywords = "Bacillibactin, Bacillus anthracis, Iron, Petrobactin, Siderophores",
author = "Koppisch, {Andrew T} and Browder, {Cindy C} and Moe, {Amanda L.} and Shelley, {Jacob T.} and Kinkel, {Blaine A.} and Hersman, {Larry E.} and Srinivas Iyer and Ruggiero, {Christy E.}",
year = "2005",
month = "12",
doi = "10.1007/s10534-005-1782-6",
language = "English (US)",
volume = "18",
pages = "577--585",
journal = "BioMetals",
issn = "0966-0844",
publisher = "Springer Netherlands",
number = "6",

}

TY - JOUR

T1 - Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation

AU - Koppisch, Andrew T

AU - Browder, Cindy C

AU - Moe, Amanda L.

AU - Shelley, Jacob T.

AU - Kinkel, Blaine A.

AU - Hersman, Larry E.

AU - Iyer, Srinivas

AU - Ruggiero, Christy E.

PY - 2005/12

Y1 - 2005/12

N2 - The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.

AB - The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.

KW - Bacillibactin

KW - Bacillus anthracis

KW - Iron

KW - Petrobactin

KW - Siderophores

UR - http://www.scopus.com/inward/record.url?scp=29744459466&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29744459466&partnerID=8YFLogxK

U2 - 10.1007/s10534-005-1782-6

DO - 10.1007/s10534-005-1782-6

M3 - Article

VL - 18

SP - 577

EP - 585

JO - BioMetals

JF - BioMetals

SN - 0966-0844

IS - 6

ER -